2001 Fiscal Year Final Research Report Summary
Antiprotease of pulmonary fibrosis Using Micro Two-Dimensional Electorophoretic Analysis
| Project/Area Number |
11660318
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Azabu University |
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Principal Investigator |
SAKAGUTI Kazuko Azabu University, Environmental Health, assistant professor, 環境保健学部, 講師 (40153879)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Jun Azabu University, Environmental Health, professor, 環境保健学部, 教授 (20075930)
AKAHORI Fumiaki Azabu University, Veterinary Medicine, professor, 獣医学部, 教授 (70063964)
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| Project Period (FY) |
1999 – 2001
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| Keywords | pulumonary fibrosis / antiprotease / micro two-dimensional electrophoretic (M2D-PAGE) analysis / proteome analysis / rat / rabbit |
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| Research Abstract |
We examined antiprotease (specifically, a_2-macrogrobulin : a_2-M) and ceruloplasmin (Cp) employing micro two-dimensional electrophoretic (M2D-PAGE) analysis to determine the characteristics of paraquat poisoned rats and rabbits that were not exhibiting paraquat induced pulmonary fibrosis.
Rats were subjected to subacute exposure to PQ (at a dose of 7 mg/kg by s.c. administration once a day on 6-9 settings of administration). They were divided into a group of death from subacute toxicity, a group of rats with recovery from subacute toxicity and progression to pulmonary fibrosis, and a group of rats resistant to PQ. The rats with the fatal outcome from subacute toxicity showed a remarkable increase in α_2M-1. The rats, which escaped death from subacute toxicity and showed progression to pulmonary fibrosis, showed remarkable decreases in α_2M-2, α_2M-3, and α_2M-4 and α_1AT. The rats that were resistant to PQ showed relative increases in all molecular species of α_2M. Particularly the increases in major α_2M molecular species, α_2M-1 and α_2M-4, and a minor molecular species, α_2M-3, were characteristically observed in the rats.
The rabbits with subacute PQ toxicity exhibited an increase in the early stage followed by a decrease in the later stage of the experimental reriod for the α_2M-1. In additon, a delayed increase in the α_2M-2 throughout the experimental period was documented. Our result indicate that the biological significance of α_2M components (α_2M-1 and α_2M-2) in the rabbit may constitute a species difference for PQ toxicity.
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