2000 Fiscal Year Final Research Report Summary
Molecular biological and cytochemical study on the mechanisms of expression of steroid hormone synthesizing enzymes.
| Project/Area Number |
11670013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | The University of Tokushima |
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Principal Investigator |
ISHIMURA Kazunori The University of Tokushima, School of Medicine, Department of Anatomy, Professor, 医学部, 教授 (90112185)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Steroid hormones / 17β-Hydroxysteroid dehydrogenase / 17α-Hydroxylase / C17-20 lyase / 5α-Reductase / Cytochemistry / Electron microscopy / Prostaglandin / Spinal cord |
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| Research Abstract |
(1) We got polyclonal antibodies against 3β-and 17β-hydroxysteroid dehydrogenases. They are applicable for immunohistochemistry.
(2) 17α-Hydroxylase/C17-20 lyase was detected in rat gastric parietal cells and pituitary intermediate lobe cells by means of immunohistochemistry and Western blotting.
(3) Aromatase, 17β-hydroxysteroid dehydrogenase and 5α-reductase were immunohistochemically detected in rat olfactory bulb.
(4) Dexamethasone enhances serum deprivation-induced necrotic death of rat C6 glioma cells through activation of glucocorticoid receptors. The appearance of 5α-reductase immunoreactivity in the cytoplasm of C6 glioma cells under serum-free conditions was probably not due to changes in 5α-reductase gene expression.
(5) Liver type prostaglandin F (PGF) synthase was immunocytochemically localized in the sinusoidal endothelial cells of rat liver.
(6) In the rat spinal cord, lung type PGF synthase was immunolocalized in the dendrites of nerve cells and vascular endothelial cells, and liver type one in the tanycytes and vascular endothelial cells.
(7) We could not set good antibody against cytochrome b5 which plays important roles in the contorl of function of 17α-hydroxylase/C17-20 lyase.
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Research Products
(20 results)
