2000 Fiscal Year Final Research Report Summary
Ontogeny and differentiation of oligodendrocytes in the central nervous system using the antibody recognizing immature oligodendrocytes.
| Project/Area Number |
11670016
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
TSURUO Yoshihiro Wakayama Medical University Medical School, Professor, 医学部, 教授 (90207449)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRASAWA Nobuyuki Wakayama Medical University Medical School, Associate professor, 医学部, 助教授 (40133392)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Oligodendrocyte / Rat / Central nervous system / Brain / Spinal cord / Immunohistochemistry / Ontogeny / Differentiation |
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| Research Abstract |
The ontogeny and differentiation of oligodendrocytes were examined immunohistochemically with the mouse monoclonal antibodies (clones 4F2 and 12F7) that were generated using a huge amount of homogeneous population of stage-specific immature oligodendrocytes as a immunogen, in the rat central nervous system including the prosencephalon and the spinal cord during the development from the early embryonal to the adult period. The cells immunoreactive for the antibody of clone 4F7 were observed in the neuroepithelium lining the ventricles and the neighboring ventricular zone in the early embryonal days (E9-E10). They were widely distributed through the brain and spinal cord. The 4F7-positive cells were not immunostained with the TUJ1 antibody that recognizes the immature neurons, suggesting that they are the progenitors to generate the oligodendrocytes, but not neurons. With development, these cells were located th roughout the wall of the brain from the ventricular zone beneath the pia mater, and were preferably in the white matter rather than the gray matter after birth. Their processes were arborized in the neonatal period, and in adults fine radiated processes were elongated to envelop the axons. The 4F7-positive cells were labeled with several oligodedroglial markers in adults. They were observed in the subventricular zone at the dorsolateral corner of the lateral ventricle in adults and senility, suggesting the presence of immature oligodendrocytes. Another clone (12F7) showed the immunoreactivity in the myelin part of the white matter only after birth. Within the white matter, all of the myelin did not show the 12F7 immunoreactivity.
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