2000 Fiscal Year Final Research Report Summary
コレシストキニンA受容体欠損ラットの病態と適応 副題:自然発症CCK-A受容体遺伝子欠損動物および遺伝子ターゲッテイング動物による検討
| Project/Area Number |
11670079
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Tokyo Meteropolitan Institute of Gerontology |
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Principal Investigator |
KANAI Setuko TOKYO METROPOLITAN INSTITUTE OF GERONTOLOGY : DEPT.CLINICAL PHYSIOLOGY, ASSISTANTS RESEARCHER, 臨床生理部門, 研究助手 (90100122)
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| Co-Investigator(Kenkyū-buntansha) |
MASUDA Masao TOKYO METROPOLITAN INSTITUTE OF GERONTOLOGY : DEPT.CLINICAL PHYSIOLOGY, ASSISTANTS RESEARCHER, 臨床生理部門, 研究助手 (20260284)
MIYASAKA Kyouko TOKYO METROPOLITAN INSTITUTE OF GERONTOLOGY : DEPT.CLINICAL PHYSIOLOGY, HEAD, 臨床生理部門, 研究部門長 (90166140)
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| Project Period (FY) |
1999 – 2000
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| Keywords | CCK / adaptation / environment / CCK-A receptor |
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| Research Abstract |
We examined gastric acid secretion in naturally occurring CCK-A receptor gene knockout rats. Gastric acid secretion stimulated by gastrin or histamine was significantly higher in CCK-A receptor gene knockout rats than those of control rats. The levels of CCk-B/gastrin receptor mRNA in the stomach of CCK-A receptor gene knockout rats were 2-fold higher than those of control rats, although those of H+/K+-ATPase and somatostatin mRNA were not different. Histological examination reveald thickening of the mucosa of the glandular stomach, and hyperplasia and hypertrophy of parietal cells in CCK-A receptor gene knockout rats although immunohistochemistry of gastrin and somatostatin revealed the similar staining and the plasma level of gastrin were comparable for both strains. Increased acid secretion in CCK-A receptor gene knockout rats is considered to be due to hyperplasia and hypertrophy of parietal cells.
We also examined gastric emptying in CCK-A receptor gene knockout rats. Gastric emptying was significantly reduced in CCK-A receptor gene knockout rats compared with that in control rats. The prior treatment of reserpine significantly enhanced gastric emptying in both strains. However, higher dose of reserpine was required in CCK-A receptor gene knockout rats to induce a similar effect to control rats. It is suggested that the delayed gastric emptying in CCK-A receptor gene knockout rats may be due to inceased sympathetic nerve function.
The experiments using CCK-A receptor gene knockout mice as now in progress.
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Research Products
(32 results)
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[Publications] Miyasaka K., Shinozaki H., Suzuki S., Sato Y., Kanai S., Masuda M., Jimi A., Nagata A., Matsui T., Noda T., Kono A.and Funakoshi A.: "Disruption of cholecysotkinin (CCK)-B receptor gene did not modify bile or pancreatic secretion or pancreatic growth. A study in CCK-A receptor gene knockout mice."Pancreas. 19. 114-118 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shimokata H, Yamada Y, Nakagawa M, Okubo R, Saido T, Funakoshi A, Miyasaka K, Ohta S, Tsujimoto G, Tanaka M, Ando F, Niino N: "Distribution of geriatric disease-related genotypes in the National Institute of Longevity Sciences, Longitudinal Study of Anging (NILS-LSA)."J Epidemiology. 10. S46-S55 (2000)
Description
「研究成果報告書概要(欧文)」より
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