2000 Fiscal Year Final Research Report Summary
Study on Activated Prenylated Derivertives-Containing Liposomes Controllable for Switching of Cell Cycle
| Project/Area Number |
11670123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
YASUDA Tatsuji Okayama University, Medical School, Professor, 医学部, 教授 (30092357)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Cell cycle / liposome / cholesterol synthesis / small G protein / geranylgeranyl pyrophosphate synthase / gene cloning |
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| Research Abstract |
The major purpose of the present study is to clarify the biological significance of prenylation of proteins associated with intracellular transduction and the mechanism on prenylation.
To analysis on the roles of isopenoide compounds, as an intermediated metabollite of the cholesterol synthesis pathway, we developed a novel method to incorporate those intermediates-encapslated liposomes into cells rested by inhibiting HMG-CoA reductase, as a rate-limiting enzyme in the cholesterol synthesis pathway. This method provided the observation that geranylgeranyl pyrophosphate (GGPP), one of isoprenoid compounds, has a role on hormone-dependent DNA synthesis in thyroid cells and results in regulating those cell cycle. It was also observed that GGPP is a critical compound for cortical nuerons to protect cell death.
We also cloned cDNA of GGPP synthase by detecting a function assay of genes for lipid metabolism enzymes, using E.coli.
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