2000 Fiscal Year Final Research Report Summary
TISSUE FACTOR PATHWAY INHIBITOR IN VASCULAR LESION AND TUMOR TISSUE
| Project/Area Number |
11670177
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Miyazaki Medical College |
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Principal Investigator |
ASADA Yujiro MIYAZAKI MEDICAL COLLEGE, PATHOLOGY, PROFESSOR, 医学部, 教授 (70202588)
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| Co-Investigator(Kenkyū-buntansha) |
SUMIYOSHI Akinobu MIYAZAKI MEDICAL COLLEGE, VICE PRESIDENT, 医学部, 副学長 (80038695)
MARUTSUKA Kousuke MIYAZAKI MEDICAL COLLEGE, PATHOLOGY, RESEARCH ASSOCIATE, 医学部, 助手 (00239154)
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| Project Period (FY) |
1999 – 2000
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| Keywords | TISSUE FACTOR / TISSUE FACTOR PATHWAY INHIBITOR / CORONARY ATHEROSCELROSIS / COLON CANCER / LUNG CANCER |
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| Research Abstract |
(1) Atherectomy specimens taken from patients with unstable angina were examined by immunohistochemistry. Smooth muscle cells (SMCs) and macrophages showed positive staining for tissue factor (TF) and TF pathway inhibitor (TFPI). Staining intensity for TFPI was poor in SMCs in proliferative phase and strong in endothelial cells of microvessels. To elucidate the role of TFPI in vascular wall, TFPI gene transfer in vascular SMCs was performed by adenovirus vector. Migratory activity of TFPI overexpressed SMCs was decreased, and thrombus formation and neointimal growth were markedly inhibited in TFPI overexpressed carotid arteries. These results suggest that TFPI immunostaining of atherectomy specimens would be useful for prediction of thrombosis and/or restenosis.
(2) Cancer tissues (colon cancer and lung cancer) were also examined by immunohistochemistry. There was no correlation between cancer cell differentiation and staining intensity for TFPI.In colon cancer tissues, cancer cells in metastatic lesions were stained positive for TFPI more intensively than those is primary lesions. These results suggest that TFPI may be involved in cancer metastasis.
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[Publications] Sato, Y., Kataoka, H., Asada, Y., Marutsuka, K., Kamikubo, Y., Koono, M.and Sumiyoshi, A.: "Overexpression of tissue factor pathway inhibitor in aortic smooth muscle cells inhibits cell migration induced by tissue factor/factor VIIa complex."Thromb.Res.. 94. 401-406 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Nishida, T., Ueno, H., Atsuchi, N., Kawano, R., Asada, Y., Nakahara, Y., Kamikubo, Y., Takeshita, A.and Yasui, H.: "Adenovirus-mediated local expression of human tissue factor pathway inhibitor eliminates shear stress-induced recurrent thrombosis in the injured carotid artery of the rabbit."Circ.Res.. 84. 1446-1452 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Atsuchi, N., Nishida, T., Marutsuka, K., Asada, Y., Kamikubo, Y., Takeshita, A., and Ueno, H.: "Combination of a brief irrigation with tissue factor pathway inhibitor (TFPI) and adenovirus-mediated local tfpi gene transfer additively reduces neointima formation in balloon-injured rabbit carotid arteries."Circulation. 103. 570-575 (2001)
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「研究成果報告書概要(欧文)」より
