2000 Fiscal Year Final Research Report Summary
Molecular Mechanisms of the promotion of lymphomagenesis by inflammation
| Project/Area Number |
11670212
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | HOKKAIDO UNIVERSITY (2000)
Osaka University (1999) |
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Principal Investigator |
KANNO Hiroyuki Hokkaido Univ. Institute for Genetic Medicine, Assoc.Prof., 遺伝子病制御研究所, 助教授 (40252663)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAKUWA Tetsuya Osaka Univ. Grad.School of Medicine, Inst., 医学系研究科, 助手 (40244933)
MIWA Hideaki Osaka Univ. Grad.School of Medicine, Inst., 医学系研究科, 助手 (30294078)
AOZASA Katsuyuki Osaka Univ. Grad.School of Medicine, Prof., 医学系研究科, 教授 (30115985)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Malignant lymphoma / Inflammation / Cytokine / Reactive oxygen species / Apoptosis / Genetic lesions |
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| Research Abstract |
1.The xanthine (X) /xanthine oxidase (XOD) system serves as an in vitro source of reactive oxygen species and apoptosis of lymphoblastoid cell lines (LCLs) was induced with X/XOD treatment. Among cytokines such as interleukin (IL)-6, IL-10 and interferon-gamma, only pretreatment with IL-6 gave LCLs the resistance to X/XOD-induced apoptosis. Furthermore, the LCLs repeatedly treated with X/XOD and cultured with or without IL-6 showed much more structural abnormalities of chromosomes than those without X/XOD treatment. Colony forming efficiency of X/XOD-treated LCLs with IL-6 was significantly higher than those without IL-6, and even relatively higher than LCLs without X/XOD treatment.
2.The cloning procedures of these LCLs on the soft agar plates were performed and 5 to 10 clones in each sublines were established. No mutations were detected in exon 5,6,7 and 8 of p53 gene of these clones by screening with PCR-SSCP methods.
3.Approximately twenty cases of malignant lymphoma of thyroid gland were collected and the following studies were performed :
(1) Microsatellite instability (MSI) was detected in high-grade diffuse large B-cell lymphomas at relatively higher frequency, although low-grade MALToma did not show MSI.
(2) Among several cytokines affecting B cell growth, only interleukin-7 showed the difference in expression between thyroid lymphoma and chronic lymphocytic thyroiditis by RT-PCR analysis.
(3) By using the methylation-specific PCR, the methylation status of death-associated protein-kinase CpG island in its promoter region was examined. The frequency of methylation was higher in thyroid lymphoma cases than in clonic lymphocytic thyroiditis cases.
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[Publications] Takakuwa, T., Hongyo, T., Syaifudin, M., Kanno, H., Matsuzuka, F., Narabayashi, I., Nomura, T., and Aozasa, K.: "Microsatellite instability and k-ras, p53 mutations in thyroid lymphoma"Japanese Journal of Cancer Research. 91(3). 280-286 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Miwa, H., Kanno, H., Munakata, S., Akano, Y., Taniwaki, M., and Aozasa, K.: "Induction of chromosomal aberrations and growth-transfor mation of lymphoblastoid cell lines by inhibition of reactive oxygen species-induced apoptosis with interleukin-6"Laboratory Investigation. 80(5). 725-734 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Nakatsuka, S., Takakuwa, T., Tomita, Y., Miwa, H., Matsuzuka, F., and Aozasa, K.: "Role of hypermethylation of DAP-kinase CpG island in the development of thyroid lymphoma"Laboratory Investigation. 80(11). 1651-1655 (2000)
Description
「研究成果報告書概要(欧文)」より
