2000 Fiscal Year Final Research Report Summary
Analysis of Tumor Rejection Antigen of Murine Myeloma MOPC70A Recognized by Specific Cytotoxic T cell.
| Project/Area Number |
11670216
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
UENAKA Akiko Okayama University, Medical School, Assistant, 医学部, 助手 (50273967)
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| Co-Investigator(Kenkyū-buntansha) |
ONO Toshiro Okayama University, Medical School, Assistant, 医学部, 助手 (50185641)
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| Project Period (FY) |
1999 – 2000
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| Keywords | murine myeloma MOPC70A / cytotoxic T cell (CTL) / antigen presenting molecule / anitgen gene / expression cloning / epitope |
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| Research Abstract |
Murine myeloma MOPC70A specific cytotoxic T cell (CTL) clone E10 was established from spleen cells of tumor bearing BALB/c mouse. 2. Expression cDNA library was cloned from mRNA of MOPC70A into mammalian expression vector pMET7 and divided into pools of 100 bacterial colonies. And the total 56,000 cDNA clones were tested for their ability to stimulate TNFα production by E10 CTL after transfection into COS7 transfected H-2 gene. 3. A clone (2D2) which stimulate E10 to produce TNFα and IFNγ was cloned. 4. The sequence of 2D2 provided 1398 bp long. Sequence homology analysis revealed that 2D2 is murine apoptosis inducing factor (mAIF). 5. To identify antigenic CTL epitope encoded by the 2D2 gene, the ability of deletion mutant of 2D2 gene to stimulate E10 to produce TNFα were investigated. The results suggested that the antigenic epitope resided within the region of 737-870bp of 2D2. From analysis of the synthetic candidate peptide on the region of 737-870 bp, a nonamer peptide DLGPDVGYEA sensitized E10 CTL was found as an epitope.
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[Publications] Ishii, T., Udono, H., Yamano, T., Ohta, H, Uenaka, A., Ono, T., Hizuta, A., Tanaka, N., Srivastava, P.K.and Nakayama, E.: "Isolation of MHC class I-restricted tumor antigen peptide and its precursors associated with heat shock proteins hsp70, hsp90 and gp96."J.Immunol.. 162. 1303-1309 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Matsuo, M., Wada, H., Honda, S., Tawara, I., Uenaka, A., Kanematsu, T.and Nakayama, E.: "Expression of multiple unique rejection antigens on murine leukemia BALB/c RL♂1 and the role of dominant Akt antigen for tumor escape."J.Immunol.. 162. 6420-6425 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Tanino, M., Matsuo, M., Uenaka, A., Tsukuda, K., Ouchida, M., Nakayama, E.: "Transforming activity of the RL-akt gene, a c-akt gene activated by long terminal repeat insertion in murine leukemia RL♂1 cells"Molecular Carcinogenesis. 26. 286-297 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Kalergis, A.M., Ono, T., wang, F., DiLorenzo, T.P., Honda, S., and Nathenson, S.G.: "Single amino acid replacements in an antigenic peptide are sufficient alter the TCR Vβ repertore of the responding CD8^+ cytotoxic lymphocyte population."J.Immunol.. 162. 7263-7270 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Ono, T., Sato, S., Kimura, N., Tanaka, N., Shibuya, A., Old, L.J.and Nakayama, E.: "Serological analysis of BALB/c methylcholanthrene sarcoma Meth A by SEREX.Identification of a cancer/testis antigen."Int.J.Cancer. 88. 845-851 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Ono, T., Kurashige, T., Harada, S., Noguchi, Y., Saika, T., Niikawa, N., Aoe, M., Nakamura, S., Higashi, T., Hiraki, A., Wada, H., Kumon, H., Old, LJ.and Nakayama, E.: "Identification of proacrosin binding protein sp32 precursor as a new human cancer/testis antigen."Proc.Natl.Acad.Sci.USA. 98. 3282-3287 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Yamamoto, T., Okano, M., Ono, T., Nakayama, E., Yoshino, T., Satoskar, A.R., Harn Jr.A.D.and Nishizaki, K.: "Gender-related differences in the initiation of allergic rhinitis in mice."Allergy. (in press).
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「研究成果報告書概要(欧文)」より
