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2000 Fiscal Year Final Research Report Summary

Basic and chinical research for cancer therapy and dendritic cells

Research Project

Project/Area Number 11670438
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionThe University of Tokyo

Principal Investigator

TAKAMIZAWA Masaru  Faculty of Medicine Balanch Hosp. The University of Tokyo Assitant Profe, 医学部・附属病院・分院, 講師 (40216784)

Co-Investigator(Kenkyū-buntansha) IWATA Tsutomu  Faculty of Medicine Balanch Hosp. The University of Tokyo Associate Profe, 医学部・附属病院・分院, 助教授 (00134578)
Project Period (FY) 1999 – 2000
Keywordsdendritic cells / vaccination / lysate / rhabdomyo sarcoma / synovial sarcoma / IL-2 / PPD / prevention
Research Abstract

Recently, vaccination therapy with dendritic cells (DC) was widely applied for cancer patients, and its effectiveness was demonstrated in some cases of malignant melanoma, malignant lymphoma (B cell type), renal cancer, prostatic cancer, lung and colon cancer. However, this therapy has not been established in children, yet. It is well known that the prognosis of malignant tumor, especially sarcoma in children is very poor. We have treated terminal stage of patients, 9-year-old girl with rhabdomyosarcoma and 15-year-old girl with synovial sarcoma by vaccination with tumor lysate and dendritic cells. 300ml of peripheral blood was drawn from the patients. 20 ml buffy coat was taken from them and the remaining was returned to the patient, using apheresis bag. Peripheral blood mononuclear cells were isolated by Ficoll gradient separation, and divided into two populations, plastic non-adherent and adherent cells. The former cells were cultured with AIM-V medium containing10% human serum for 36 h in the presence of tumor lysate. DC were isolated by metrizamide gradient separation method, and injected into a patient with PPD pulsed monocytes and rIL-2. The latter cells were cultured with rGM-CSF and rIL-4 in the presence of tumor lysate for 7days. Monocyte derived DC were harvested and injected into a patient with rIL-2. This therapy was done every 2-4 weeks interval. Results : Vaccination therapy didn't affect on the clinical course of rhabdomyosarcoma patient at all. On the other hand, it showed partial response in the patient with synovial sarcoma, demonstrating the disappearance of pleural effusion and cough. New metastatic tumors were not detected for nearly 1 year after immunotherapy, although only one lung tumor was still growing slowly. She lives her usual daily life without difficulties. Vaccination therapy may be effective on the prevention of metastasis in an early stage of synovial sarcoma.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Takuya Osada: "Activation of cunti-tumor immunity by dendritic cells in a patient with malignant intracranial germ cell tumor"Japanese Jouroal of Clinical oncology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高見沢勝: "ヒト末梢血樹状細胞による免疫療法"日本リンパ網内系学会誌. 40. 27-28 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高見沢勝: "樹状細胞-基礎から臨床へ(分担)"南江堂. 188 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takuya Osada et al.: "activation of anti-tumor immunity try dendritic cells in a patient with malignant intracramial germ cell tumor."Japanese Journal of Clinical oncology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masarn Takanizawa et al.: "Immunothoorapy for caner patient with peripheral blood dendritic cells.(in Japanese)"Nihon RINpanounaikei gakkaishi. 40. 27-28 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masaru Takamizawa: "Dendritic cells ; Basis and clinical application (in Japanese)"Nan Koudou (publisher).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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