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2000 Fiscal Year Final Research Report Summary

Secretory IgA-mediated basophil activation. Its mechanisms and implication in allergy.

Research Project

Project/Area Number 11670440
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionUniversity of Tokyo

Principal Investigator

YAMAGUCHI Masao  University of Tokyo Hospital Assistant professor, 医学部・附属病院, 助手 (10302704)

Co-Investigator(Kenkyū-buntansha) HIRAI Koichi  University of Tokyo, Graduate School of Medicine, Associate professor, 大学院・医学系研究科, 客員助教授 (10156630)
Project Period (FY) 1999 – 2000
Keywordssecretory IgA / basophils / allergy
Research Abstract

Secretory IgA (sIgA) is the most abundant Ig isotype in mucous secretions in the upper and lower airways, where basophils exert effector functions during allergic inflammation. We recently demonstrated that immobilized sIgA on Sepharose beads is capable of inducing basophil degranulation (〜15% of total histamine). To investigate the detailed mechanisms of this degranulation, we established in this study a new assay system for sIgA-mediated basophil activation. Immobilized sIgA on a plastic surface induced strong histamine release (〜50% of total histamine) comparable to anti-IgE, and we analyzed the nature of basophil signal transduction by sIgA using various inhibitors. sIgA-induced basophil histamine release was inhibited completely by pertussis toxin, but anti-IgE-induced release was not affected. sIgA-mediated release was also inhibited by phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor, wortmannin. These results strongly suggest that sIgA activates basophils via an IgE-independent novel mechanism involving both Gi protein and PI 3-kinase.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Iikura M et al.: "Secretory IgA-mediated basophil activation"Biochem Biophys Res Commun. 264. 575-579 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada H et al.: "Eotaxin in induced sputum of asthmatics Relationship with eosinophils and eosmophil cationic protein in sputum"Allergy. 55. 392-397 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iikura M et al.: "Regulation of surface FcεR1 expression on human eosinophils by IL-4 and IgE"Int Arch Allergy Immunol. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirai K et al.: "Mast cells and basophils"Academic Press. 11 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iikura M, Yamaguchi M,Miyamasu M, Morita Y, Iwase T, Moro I, Yamamoto K, Hirai K.: "Secretory IgA-mediated basophil activation."Biochemical and Biophysical Research Communications. 264. 575-579 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada H, Yamaguchi M, Nakajima T, Morita Y, Yamamoto K, Sano Y, Hirai K.: "Eotaxin in induced sputum of asthmatics. Relationship with eosinophils and eosinophil cationic protein in sputum."Allergy. 55. 392-397 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iikura M, Yamaguchi M, Hirai K, Miyamasu M, Yamada H, Nakajima T, Fujisawa T, Ra C, Morita Y, Yamamoto K.: "Regulation of surface FcεRI expression on human eosinophils by IL-4 and IgE."International Archives of Allergy and Immunology.. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirai K, Iikura M, Miyamasu M, Yamaguchi M.: "Chapter 14. Interaction between secretory IgA and human basophils. Mast cells and basophils."Academic Press.. 209-219 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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