2000 Fiscal Year Final Research Report Summary
Functional analysis of HCV core protein using HCV core transgenic mouse
| Project/Area Number |
11670476
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Chiba University |
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Principal Investigator |
IMAZEKI Fumio Chiba University, School of Medicine, Lecturer, 医学部, 講師 (40223325)
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| Co-Investigator(Kenkyū-buntansha) |
TOKUHISA Takeshi Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (20134364)
HONDA Arata Chiba University, University Hospital, Researcher, 医学部, 医員
YOKOSUKA Osamu Chiba University, School of Medicine, Lecturer, 医学部, 講師 (90182691)
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| Project Period (FY) |
1999 – 2000
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| Keywords | HCV core protein / transgenic mouse / suppression subtractive hybridization / cDNA array / DNA chip / mRNA |
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| Research Abstract |
We analyzed comprihensively the difference of mRNAs expressed in the livers of HCV core transgenic mouse and wild mouse in order to clarify the function of HCV core protein in vivo by the method of subtraction and cDNA microarray. We have got fifty clones of HCV core transgenic mouse subtracted from wild one and 44 clones of wild mouse subtracted from HCV core transgenic one by the method of suppression subtractive hybridization. Sequence analysis of these clones showed not any genes associated with inflammation or carcinogenesis, and the intensity of expression of these genes between transgenic and wild ones was within two times. Next, we analyzed the difference of expression of about 1000 genes between transgenic and wild mice by cDNA array and DNA chip, respectively. Both methods revealed several genes expressed more than two times in transgenic mouse, and these genes seem also not associated with inflammation or carcinogenesis. We will analyze the significance of these genes from now.
Expression of HCV core mRNA was ascertained in the transgenic mouse used in this study by northern blotting and the amount of mRNA was estimated to be around 2 pg/μgRNA in the liver. HCV core protein was not analyzed in the taransgenic mouse used in this study, but previous results showed the expression of HCV core protein by western blotting. Therefore HCV core protein might have little effect on the expression of genes in this transgenic mouse in vivo.
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Research Products
(2 results)
