2001 Fiscal Year Final Research Report Summary
Analysis of oncogenesis in pancreatic cancer using ductal cells tranfered with papilloma virus E6/E7
| Project/Area Number |
11670481
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KAWABE Takao Faculty of Medicine (Hospital), The University of Tokyo, Lecturer, 医学部・附属病院, 講師 (40195136)
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| Co-Investigator(Kenkyū-buntansha) |
大橋 誠 東京大学, 医学部・附属病院, 医員
TADA Minoru Faculty of Medicine (Hospital), The University of Tokyo, Associate, 医学部・附属病院, 助手 (80302719)
TADA Minoru Faculty of Medicine (Hospital), The University of Tokyo, medical staff
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| Project Period (FY) |
1999 – 2000
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| Keywords | Papilloma virus / Gene transfer / Pancreatic duct cell / Pancreatic cancer |
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| Research Abstract |
1. Monolayer culture of pancreatic duct cells. We established the monolayer culture of rat pancreatic duct cells. Then we also tried to culture of human pancreatic ductal cells, but it was not successful. We did not obtain a large amount of human materials and the materials were not brought to us soon after they were obtained from the patients. These were considered part of the reasons for our failure in human pancreatic duct cell culture.
2. Construction of recombinant adenovirus vectors. Bcl-2 and bcl-XL are considered to be anti-apoptotic genes and mutated k-ras to promote cell growth. We tried to construct adenovirus vectors integrated with these genes using 293 cells by COS-TCP method.
The expression of Bcl-XL was confirmed by Western blotting in the cells infected with recombinant adenovirus. An elevation of the bio-activity was also revealed by the anti-apoptotic effects of Bcl-XL, which was accessed by resistant property to Cisplatin.
The clones of the recombinant virus integrated with bcl-2 or mutated k-ras were obtained. However, the amount of the virus titer was not enough for further experiments in 293 cells. To obtain enough amount, another technique, such as CRE-loxP or conditional expression system, may be needed in further investigations.
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Research Products
(12 results)
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[Publications] Ikenoue T, Togo G, Nagai K, Ijichi H, Kato J, Yamaji Y, Okamoto M, Kato N, Kawabe T, Tanaka A, Matsumura M, Shiratori Y, Omata M.: "Frameshift mutations at mononucleotide repeats in RAD50 recombinational DNA repair gene in colorectal cancers with microsatellite instability"Jpn J Cancer Res. 92. 587-91 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Obi S, Shiratori Y, Shiina S, Hamamura K, Kato N, Imamura M, Teratani T, Sato S, Komatsu Y, Kawabe T, Omata M.: "Early detection of haemobilia associated with percutaneous ethanol injection for hepatocellular carcinoma"Eur J Gastroenterol Hepatol. 12. 285-90 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Maeda S, Yoshida H, Ogura K, Yamaji Y, Ikenoue T, Mitsushima T, Tagawa H, Kawaguchi R, Mori K, Mafune K, Kawabe T, Shiratori Y, Omata M.: "Assessment of gastric carcinoma risk associated with Helicobacter pylori may vary depending on the antigen used : CagA specific enzyme-linked immunoadsorbent assay (ELISA) versus commercially available H. pylori ELISAs"Cancer. 88. 1530-5 (2000)
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[Publications] Ohashi M, Kanai F, Ueno H, Tanaka T, Tateishi K, Kawakami T, Koike Y, Ikenoue T, Shiratori Y, Hamada H, Omata M.: "Adenovirus mediated p53 tumour suppressor gene therapy for human gastric cancer cells in vitro and in vivo"Gut. 44. 366-71 (1999)
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「研究成果報告書概要(欧文)」より
