2000 Fiscal Year Final Research Report Summary
Association of hepatic oval cell and liver diseses : implication to new therapy
| Project/Area Number |
11670504
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Tottori University |
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Principal Investigator |
KAWASAKI Hironaka Tottori University, Faculty of Medicine, Second Department of Internal Medicine, Professor, 医学部, 教授 (60108826)
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| Co-Investigator(Kenkyū-buntansha) |
SHIOTA Goshi Tottori University, Faculty of Medicine, Second Department of Internal Medicine, Assistant Professor, 医学部・附属病院, 講師 (70263457)
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| Project Period (FY) |
1999 – 2000
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| Keywords | hepatic oval cell / fulminant hepatitis / HGF / gene therapy / signal transduction |
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| Research Abstract |
To clarify the effect of hepatocyte growth factor (HGF) on proliferation of hepatic oval cells, we transferred HGF gene into liver of the Solt- Farber rat model. Male Fisher 344 rats were infected with a recombinant adenovirus carrying the cDNA for HGF (pAxCAHGF) from tail vein. HGF mRNA showed its peak at 4 days, and diminished thereafter. The total and proliferating cell nuclear antigen-positive hepatic oval cells were significantly elevated in HGF-transferred rats, in which stem cell factor and c-kit mRNA increased increased at each time point. The results suggest that in vivo transfer of hepatic oval cells in the Solt-Farber model in rats.
Recently, we examined the effect of pAxCAHGF on OC/CDE6 cells, hepatic oval cells. The OC/CDE6 cells, which are infected by pAxCAHGF, grow more rapidly than those by pAXCALacZ.The pathway via which signal of HGF is transmitted is mainly PI3-K, especially Akt. The MAPK is associated with the signal transduction of HGF, but not a main pathway.
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[Publications] Goshi Shiota, Takahiro Kunisada, Kenji Oyama, Akihide Udagawa, Takahiro Nomi, Kiwamu Tanaka, Atsushi Tsutsumi Nakamura, Hirofumi Mamada, Takashi Sakatani: "Stewaqrt Sell, Kenzo Sato, Hisao Ito, Hironaka Kawasaki. In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cell in a 2-acetylaminofluorene/partial hepatectomy model in rats."FEBS Letters. 470. 325-330 (2000)
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