2000 Fiscal Year Annual Research Report

ECL細胞における胃粘膜増殖因子Regの産生調節機構の解明

Research Project

Project/Area Number	11670505
Research Institution	Shimane Medical University
Principal Investigator	木下芳一島根医科大学, 医学部, 教授 (30243306)
Keywords	胃 / Reg / CINC-2β / ガストリン / ラット
Research Abstract	私共は、胃粘膜の増殖因子であるReg蛋白が胃粘膜のECL細胞で産生されていることを明らかとしてきた。本研究では、Reg蛋白がどのような発現調節をうけているかをラットを用いたin vivoとラット胃粘膜より分離したECL細胞を用いたin vitroの検討を行った。in vivoの検討では、胃粘膜損傷のない場合にはガストリンがReg蛋白の発現を促進させていた。一方、胃粘膜に損傷の認められる時にはReg蛋白の発現はガストリンではなくCXCケモカインの一つであるCINC-2βによって促進されていることが明らかとなった。単離ECL細胞を用いた検討では、ガストリンとCINC-2βはそれぞれ独立してReg蛋白の発現を促進することが明らかとなった。また、ECL細胞にはガストリン受容体、CXCケモカインの受容体であるCXCR1が発現していること、胃粘膜の増殖細胞帯の増殖細胞にはReg蛋白の受容体が発現していることも明らかとできた。これらの結果より正常胃粘膜の増殖のコントロールにガストリンが、損傷治癒時の増殖コントロールにはCINC-2βが大きな役割を有しており、これらがECL細胞にそれぞれの受容体を介して作用し、Reg蛋白の産生を促進し、Reg蛋白がその受容体を介して胃粘膜の増殖を促進していた。

Research Products
(24 results)

All Other

All Publications (24 results)

[Publications] Yuki T, et al: "Simultaneous elevation of serum amylase and lipase levels due to"Pancreas. 18. 418-419 (1999)
[Publications] Arima N, et al: "Predictive factors for metachronous recurrence of early gastric cancer"J.Clin.Gastroenterology. 29. 44-47 (1999)
[Publications] Ashizawa N, et al: "The morphological changes of exocrine pancreas in chronic pancreatitis."Histology and Histopathology. 14. 539-552 (1999)
[Publications] Fukuda R, et al: "Serologically silent hepatitis B virus coinfection in patients with hepatitis C"Journal of Medical Virology. 58. 201-207 (1999)
[Publications] Hirakawa K, et al: "Prevalence of non-ulcer dyspepsia in Jananese population."J Gastroenterol Hepatol. 14. 1083-1087 (1999)
[Publications] Watanabe M, et al: "Laparoscopic observations of hepatic capsular abnormalities :"Gastrointenstinal Endoscopy. 50. 664-666 (1999)
[Publications] Niigaki M, et al: "Role of hepatitis B virus in non-B, non-C chronic liver diseas"Am J Gastroenterol. 95. 239-247 (2000)
[Publications] Matsushima Y, et al: "Possible involvement of Helicobacter pylori infection in gastric ulcer"Helicobacter. 4. 94-99 (1999)
[Publications] Kinoshita Y, et al: "Mechanism of gastric mucosal proliferation induced by gastrin."Gastroenterol Hepatol. 15. D7-D11 (2000)
[Publications] Aoyama N, et al: "Helicobacter pylori-negative peptic ulcer in Japan ; Which contributes"J.Gastroenterol. 35. 33-37 (2000)
[Publications] Kinoshita Y, et al: "Esophageal mucosal acid sensitivity-normal or abnormal?"J.Gastroenterol. 35. 310-311 (2000)
[Publications] Ishihara S, et al: "Diagnostic accuracy of a new non-invasive enzyme immunoassay"Alimentary Pharmacology and Therapeutics. 14. 611-614 (2000)
[Publications] Watanabe M, et al: "Coinfection with hepatitis C virus and TT virus in a case of late onset"Am J Gastroenterol. 95. 835-836 (2000)
[Publications] Adachi K, et al: "Efficacy of sucralfate for Helicobacter pylori eradication triple therapy in"Alimentary Pharmacology and Therapeutics. 14. 919-922 (2000)
[Publications] Suetsugu H, et al: "Effect of rebamipide on prostaglandin EP4 receptor gene expression in rat"J Lab Clin Med. 136. 50-57 (2000)
[Publications] Katsube T, et al: "Helicobacter pylori infection influences nocturnal gastric acid breakthrough."Pharmacology and Therapeutics. 14. 1049-1056 (2000)
[Publications] Kawamura A, et al: "Helicobacter pylori infection and family history of gastroduodenal"Am J.Gastroenterol. 95. 2987-2988 (2000)
[Publications] Kushiyama Y, et al: "Site-dependent production of transforming growth factor-β1 in colonic"J Lab Clin Med. 136. 201-208 (2000)
[Publications] Adachi K, et al: "CYP2c19 genotype status and intragastric pH during treatment with"Alimentary Pharmacology and Therapeutics. 14. 1259-1266 (2000)
[Publications] Watanabe M, et al: "Hepatocellular carcinoma complicating HCV-negative autoimmune"J Clin.Gastroenterol. 30. 445-447 (2000)
[Publications] Kazumori H, et al: "Neutrophil chemoattractant-2β regulates the expression of the Reg Gene"Gastroenterology. 119. 1610-1622 (2000)
[Publications] Sato H, et al: "Expression of peroxisome proliferator-activated receotor (PPAR)γ"British J Cancer. 83. 1394-1400 (2000)
[Publications] Watanabe T, et al: "Effect of Helicobacter pylori infection on Zolinger-Ellison syndrome."J Gaasteoenterology. 35. 735-741 (2000)
[Publications] Adachi K, et al: "Gastroesophagesl acid reflux in elderly patients."Shimane J Med Sci. 18. 21-26 (2000)

2000 Fiscal Year Annual Research Report

ECL細胞における胃粘膜増殖因子Regの産生調節機構の解明

Principal Investigator

木下 芳一 島根医科大学, 医学部, 教授 (30243306)

Research Products

[Publications] Yuki T, et al: "Simultaneous elevation of serum amylase and lipase levels due to"Pancreas. 18. 418-419 (1999)

[Publications] Arima N, et al: "Predictive factors for metachronous recurrence of early gastric cancer"J.Clin.Gastroenterology. 29. 44-47 (1999)

[Publications] Ashizawa N, et al: "The morphological changes of exocrine pancreas in chronic pancreatitis."Histology and Histopathology. 14. 539-552 (1999)

[Publications] Fukuda R, et al: "Serologically silent hepatitis B virus coinfection in patients with hepatitis C"Journal of Medical Virology. 58. 201-207 (1999)

[Publications] Hirakawa K, et al: "Prevalence of non-ulcer dyspepsia in Jananese population."J Gastroenterol Hepatol. 14. 1083-1087 (1999)

[Publications] Watanabe M, et al: "Laparoscopic observations of hepatic capsular abnormalities :"Gastrointenstinal Endoscopy. 50. 664-666 (1999)

[Publications] Niigaki M, et al: "Role of hepatitis B virus in non-B, non-C chronic liver diseas"Am J Gastroenterol. 95. 239-247 (2000)

[Publications] Matsushima Y, et al: "Possible involvement of Helicobacter pylori infection in gastric ulcer"Helicobacter. 4. 94-99 (1999)

[Publications] Kinoshita Y, et al: "Mechanism of gastric mucosal proliferation induced by gastrin."Gastroenterol Hepatol. 15. D7-D11 (2000)

[Publications] Aoyama N, et al: "Helicobacter pylori-negative peptic ulcer in Japan ; Which contributes"J.Gastroenterol. 35. 33-37 (2000)

[Publications] Kinoshita Y, et al: "Esophageal mucosal acid sensitivity-normal or abnormal?"J.Gastroenterol. 35. 310-311 (2000)

[Publications] Ishihara S, et al: "Diagnostic accuracy of a new non-invasive enzyme immunoassay"Alimentary Pharmacology and Therapeutics. 14. 611-614 (2000)

[Publications] Watanabe M, et al: "Coinfection with hepatitis C virus and TT virus in a case of late onset"Am J Gastroenterol. 95. 835-836 (2000)

[Publications] Adachi K, et al: "Efficacy of sucralfate for Helicobacter pylori eradication triple therapy in"Alimentary Pharmacology and Therapeutics. 14. 919-922 (2000)

[Publications] Suetsugu H, et al: "Effect of rebamipide on prostaglandin EP4 receptor gene expression in rat"J Lab Clin Med. 136. 50-57 (2000)

[Publications] Katsube T, et al: "Helicobacter pylori infection influences nocturnal gastric acid breakthrough."Pharmacology and Therapeutics. 14. 1049-1056 (2000)

[Publications] Kawamura A, et al: "Helicobacter pylori infection and family history of gastroduodenal"Am J.Gastroenterol. 95. 2987-2988 (2000)

[Publications] Kushiyama Y, et al: "Site-dependent production of transforming growth factor-β1 in colonic"J Lab Clin Med. 136. 201-208 (2000)

[Publications] Adachi K, et al: "CYP2c19 genotype status and intragastric pH during treatment with"Alimentary Pharmacology and Therapeutics. 14. 1259-1266 (2000)

[Publications] Watanabe M, et al: "Hepatocellular carcinoma complicating HCV-negative autoimmune"J Clin.Gastroenterol. 30. 445-447 (2000)

[Publications] Kazumori H, et al: "Neutrophil chemoattractant-2β regulates the expression of the Reg Gene"Gastroenterology. 119. 1610-1622 (2000)

[Publications] Sato H, et al: "Expression of peroxisome proliferator-activated receotor (PPAR)γ"British J Cancer. 83. 1394-1400 (2000)

[Publications] Watanabe T, et al: "Effect of Helicobacter pylori infection on Zolinger-Ellison syndrome."J Gaasteoenterology. 35. 735-741 (2000)

[Publications] Adachi K, et al: "Gastroesophagesl acid reflux in elderly patients."Shimane J Med Sci. 18. 21-26 (2000)

木下芳一島根医科大学, 医学部, 教授 (30243306)