2000 Fiscal Year Final Research Report Summary
Pathophysiological Analysis of Prion Protein Expression in the Liver
| Project/Area Number |
11670525
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Osaka City University |
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Principal Investigator |
KAWADA Norifumi Osaka City University Medical School, Research Associate, 医学部, 助手 (30271191)
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| Co-Investigator(Kenkyū-buntansha) |
KANEDA Kenji Osaka City University Medical School, Professor, 医学部, 教授 (30161186)
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| Project Period (FY) |
1999 – 2000
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| Keywords | hepatic stellate cels / liver fibrosis / prion protein |
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| Research Abstract |
We have discovered the prion protein (PrP) gene expression in activated stellate cells by using PCR-select cDNA subtraction method. Northern blot analysis revealed that PrP mRNA was hardly expressed in quiescent stellate cells, but its expression was dramatically augmented in an activated phenotype. Western blot analysis also revealed that PrP expression was enhanced in a time-dependent manner. In in situ hybridization and in imunohistochemistry, PrP and its mRNA expression was detected in liver fibrosis model, in particular along the fibrotic septum accumulating extracellular matrix materials and smooth muscle α-actin-positive cells. In addition, PrP expression was confirmed in human diseased liver tissue with HBV and HCV-induced chronic viral hepatitis and alcoholic liver damage. PrP expression had positive relationship with inflammatory severity. Further analysis indicated that PrP expression was co-localized with the expression of smooth muscle α-actin, indicating that PrP was expressed dominantly in stellate cells. These results indicate that PrP would be utilized as a novel marker for liver fibrosis in the clinical field.
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Research Products
(17 results)
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[Publications] Okuno M, Sato T, Kitamoto T, Imai S, Kawada N, Suzuki Y, Yoshimura H, Moriwaki H, Onuki K, Masushige S, Muto Y, Friedman SL, Kato S, Kojima S.: "Increased 9,13-di-cis-retinoic acid in rat hepatic fibrosis : implication for a potential link between retinoid loss and TGF-b mediated fibrogenesis in vivo."J.Hepatol. 30. 1073-1080 (1999)
Description
「研究成果報告書概要(欧文)」より
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