2001 Fiscal Year Final Research Report Summary
Pancreatic fibrosis and diabetes in transgenic OLETF rat expressing CCK-A receptor
| Project/Area Number |
11670553
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | University of Occupational and Environmental Health |
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Principal Investigator |
NAKAMURA Makoto University of Occupational and Environmental Health, School of medicine. Third Department of Internal Medicine, Associate Professor, 医学部, 助教授 (90207902)
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| Co-Investigator(Kenkyū-buntansha) |
KIHARA Yasuyuki University of Occupational and Environmental Health, School of Medicine, Third Department of Internal Medicine, Research Associate, 医学部, 助手 (80279330)
OTSUKI Makoto University of Occupational and Environmental Health, School of Medicine, Third Department of Internal Medicine, Professor, 医学部, 教授 (00030916)
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| Project Period (FY) |
1999 – 2001
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| Keywords | Smad6 / transgenic mouse / TGF-beta / fibrosis |
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| Research Abstract |
The transforming growth factor β (TGF β) is multifunctional polypeptides that play a role in the synthesis of different extracellular matrix components such as fibronectin, proteoglycans and collagen. Recent studies have also revealed other functional properties for TGF βs including their role in various regenerative events after injury or inflammation. Inhibitory Smads (I-Smads), including Smad6 and Smad7, are characterized as cytoplasmic antagonists in the TGF β signaling pathway. I-Smads are also localized in the nucleus and Smad6 especialy can function as a transcriptional co-repressor.
To examine the effect of the overexpressed Smad6 in acinar cells of the pancreas, we planed to generate transgenic mouse expressing Smad6.Both mouse Smad6 cDNA, which from Dr Swift, were cloned in the pSI vector (Promega Corporation). Mouse Smad6 cDNA was inserted into its cloning site flanking the exon-intron organization and a polyadenlation signal of the rabbit p-globin gene. The nucleotide sequence of the constructed vector was verified by direct sequencing. We obtained 5 Smad6 transgenic mice. We would like to examine phenotypes of the Smad6 transgenic mice in the future.
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