2000 Fiscal Year Final Research Report Summary
Effects of chemokine-like proteins encoded by the human herpesvirus type 8 on HIV infection
| Project/Area Number |
11670618
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Yamanashi Medical University |
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Principal Investigator |
TERUNUMA Hiroshi Yamanashi Medical University, Department of Microbiology, Lecture, 医学部, 講師 (50217436)
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| Co-Investigator(Kenkyū-buntansha) |
IWATANI Yasumasa Yamanashi Medical University, Department of Microbiology, Assistant Professor, 医学部, 助手 (90303403)
KASAI Hirotake Yamanashi Medical University, Department of Microbiology, Assistant Professor, 医学部, 助手 (20324189)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Human herpesvirus type 8 / kaposi's sarcoma / AIDS / HIV / cehmokine / vMIP / Zamibia |
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| Research Abstract |
We studied the clinical and immunolgical characteristics of 83 Zombian patients with Kaposis sarcoma(KS)and collected biopsy samples from some of these patients after getting their informed consent. We found thet some of the KS patients still maintained high CD4 counts, but those with an oral lesion(s)had significantly lower CD4 counts. We are now determining the vMIPs nucleotide sequences from biopsy samples for comparison with those of laboratory HHV-8 strains.
To analyze the effects of chemokine-like proteins encoded by the human herpesvirus type 8 on HIV infection, we amplified and isolated viral MIP(vMIP)-I, -II, and -III genes of human herpesvirus type 8(HHV-8)from BCBL-l cell line by PCR.We then constracted these genes into an expression vector and transfected these vectors into a 293T cell line. Culture supernatant containing secreted vMIPs showed the activity of Ca2+ influx to THP-1 cell line. vMIPs genes were also introduced into Cos 7 cells and permanent cell lines secreting vMIPs have been established. We purified a large amount of vMIPs and are studying the effects of these prroteins on HIV infection and immunological effects. Similar experiments are being done using the vMIPs genes of HHV-8 isolated from KS patients.
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[Publications] Sugiura, Y., terunuma, H., Yamamoto, T., Ishikawa, M., Sato, J., Toyota, T., Iwasaki, Y.: "Host immune profiles and genotyping analysis of HIV-1 among Japanese hemophiliac patients."Fukushima Journal of Medical Science. 45. 53-62 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] He, L., Terunuma, H., Tanabe, F., Hanabusa, H., Iwamoto, A., Oka, S., Chiba, N., Kurimoto, M., Ikeda, M., Okamura, H., Ito, M.: "IL-18 and IL-1 β productions are decreased in HIV-1-seropositive haemophiliacs but not in HIV-1-seropositive non-haemophiliacs."AIDS Research and Human Retroviruses. 16. 345-353 (2000)
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[Publications] Deng, X., Terunuma, H., Handema, R., Sakamoto, M., Kitamura, T., Ito, M., Akahane, Y.: "higher prevalence and viral load of TT virus in saliva than in the corresponding serum : another possible transmission route and replication site of TT virus."J Medical Virology. 62. 531-537 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Handema, R., Terunma, H., Kasolo, F., Kasai H., Sichone, M., Mulundu, G., Deng, X., Ichiyama, K., Mitarai, S., Honda, M., Yamamoto, N., Ito, M.: "Emergence of new HIV-1 subtypes other than subtype C among antenatal woman in Lusaka, Zambia."AIDS Research and Human retroviruses. (in press).
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「研究成果報告書概要(欧文)」より
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[Publications] Owada, T., Motomura, T., Miyashita-Ogawa, Y., Kawada-Homma, M., Onishi, M., Matondo, P., Terumura, H., Numazaki, Y., Yamashita, S., Yamamoto, N.: "Antibody masking renders HIV-1 resistant to the cationic membrane filtration through alteration of its electrostatic character."J.Virological Methods. (in press).
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「研究成果報告書概要(欧文)」より
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[Publications] Dai, J.H., Iwatani, Y., Ishida, T., Terunuma, H., Kasai, H., Iwakura, Y., Fujiwara, H., Ito, M.: "Glycyrrhizin enhances IL-12 production in peritoneal macrophages"Immunology. (in press).
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「研究成果報告書概要(欧文)」より
