• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2002 Fiscal Year Final Research Report Summary

A study on the mechanism of myocardial injury in myocarditis and dilated cardiomyopathy.

Research Project

Project/Area Number 11670714
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionTokyo Women's Medical University

Principal Investigator

NISHIKAWA Toshio  Tokyo Women's Medical University, Associate Professor, 医学部, 助教授 (50120019)

Co-Investigator(Kenkyū-buntansha) ISHIYAMA Shigeru  Tokyo Women's Medical University, Assistant, 医学部, 助手 (20297542)
MASUDA Akihiro  Tokyo Women's Medical University, Associate Professor, 医学部, 助教授 (60209434)
KASAJIMA Takeshi  Tokyo Women's Medical University, Professor, 医学部, 教授 (30045653)
HIROE Michiaki  Tokyo Medical Dental University, Instructor, 医学部, 講師 (80101872)
Project Period (FY) 1999 – 2002
KeywordsDilated cardiomyopathy / chronic myocarditis / rat / tissue injury / infiltrated lymphocyte / apoptosis / Fas / Fas ligand system
Research Abstract

It has generally been recognized that myocarditis may be implicated in the pathogenesis of dilated cardiomyopathy. However, the mechanism of myocardial damage in the chronic stage of myocarditis is still unclear. In this study myocarditis was induced in Lewis rats by injection of porcine cardiac myosin and rats were harvested on day 21 or 74 after the initial injection. Apoptotic cell death was confirmed by in situ nick end labeling assay and gel-electrophoresis for DNA fragmentation on day 21, but none was disclosed on day 74. The expression of Fas mRNA and protein was detected on day 21 in the myocardium. In addition, Fas ligand was detected in some infiltrating lymphocytes. Some rats were injected with anti-Fas antibody or anti-Fas ligand antibody, and subsequently, myocardial damage was less severe than that in untreated rats on day 74. These results suggested that inhibition of apoptotic phenomenon in the acute stage may be related to the reduction of myocardial damage in the chronic stage of myocarditis.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Shigeru Ishiyama: "Inhibitory effects of vesnarinone in the progression of myocardial damage in experimental autoimmune myocarditis in rats"Cardiovasc Research. 43. 389-397 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takashi Shimjo: "Nitric oxide induces apoptotic death of cardiomyocytes via a cyclic-GMP-dependent pathway"Exp Cell Res. 247. 38-47 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshio Nishikawa: "Programmed cell death in the myocardium of arrhythmogenic right ventricular cardiomyopathy in children and adults"Cardiovasc Pathol. 8. 185-189 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshio Nishikawa: "The role of superoxide and nitric oxide in the development of myocardial injury in rat myocarditis"Microsc Microanal. 6(Suppl 2). 488-489 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshio Nishikawa: "Lymphoid cells and dendritic cells in transplanted hearts : an immunohistochemical study of three cases"Dendritic Cells. 10. 31-35 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasunari Sakomura: "Expression of proxisome proliferator-activated receptor-γ in vascular smooth muscle cells in upregulated in Marfan syndrome"Circulation. 106(Suppl I). I259-I263 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeru Ishiyama, Michiaki Hiroe, Toshio Nishikawa, et al.: "Inhibitory effects of vesnarinone in the progression of myocardial damage in experimental autoimmune myocarditis in rats."Cardiovasc Research. 43. 389-397 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takashi Shimojo, Michiaki Hiroe, Shigeru Ishiyama, Hiroshi Ito, Toshio Nishikawa, Fumiaki Marumo: "Nitric oxide induces apoptotic death of cardiomyocytes via a cyclic-GMP-dependent pathway."Exp Cell Res. 247. 38-47 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshio Nishikawa, Shigeru Ishiyama, et al.: "Programmed cell death in the myocardium of arrhythmogenic right ventricular cardiomyopathy in children and adults."Cardiovasc Pathol. 8. 185-189 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshio Nishikawa, Shigeru Ishiyama, et al.: "The role of superoxide and nitric oxide in the development of myocardial injury in rat myocarditis."Microsc Microanal. 6(suppl 2). 488-489 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshio Nishikawa, Takeshi kasajima, Akihiro Masuda, et al.: "Lymphoid cells and dendritic cells in transplanted hearts : an immunohistochemical study of three cases."Dendritic Cells. 10. 31-35 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] "Expression of proxisome proliferator-activated receptor-g in vascular smooth muscle cells is upregulated in Marfan syndrome."Circulation. 106 (suppl I). I-259-I-263 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2004-04-14  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi