2001 Fiscal Year Final Research Report Summary
Effects of Cyclophosphamide and Dioxin (TCDD) on spermatogenesis in rats
| Project/Area Number |
11670751
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Yamanashi Medical University |
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Principal Investigator |
OHYAMA Kenji Yamanashi Medical Mversity, Departait of Clinical Nursing, Professor, 医学部, 教授 (80051861)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGOMI Yoshiko Yamanashi Medical Mversity, Department of Pediatrics, Assistant, 医学部, 助手 (20198055)
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| Project Period (FY) |
1999 – 2001
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| Keywords | Growth Hormone / Spermatogenesis / Gyclophosphamide / Germ cell / Sperm / Dioxin / GH欠損ラット / ダイオキシン |
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| Research Abstract |
Effects of Cyclophosphamide and Dioxin (TCDD) on spermatogenesis in rats
1. Effects of GH on recovery from testicular damage induced by Cyclophosphamide (CP) in GH-deficient rats. CP was intravenously administered at dose of 50 mg/kg for 2 days, and rat GH was subcutaneously administered at dose of 0.3 mg/kg every day for 2 and 4 weeks. The number of spermatocytes was decreased when they exposed to CP at type A spermatogonia. The.motility index of spermatozoa was also decreased by CP. In CP/GH group, the number of those cells was recovered and the damaged motility of spermatozoa was improved. Under GH-deficient condition, exogeneous GH can improve the testicular function damaged by CP.
2. Effect of very low dose of TCDD administered to maternal rats on ganadal function of neonatal rats. TCDD was subcutaneously administered at dose of 10 Rg/kg once a week to maternal rats (7, 14, 21 dpc, and 7, 14 days after birth). 21days after birth neonatal rats were subjected. All organs were normal by macroscopic examination, and testicular tissues were also microscopically normal. Expression of Cyp lAlspecifically induced by TCDD was markedly increased in neonatal rat liver. Inhibin B (marker of Sertoli cell function) expression in testis was normal.
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