2000 Fiscal Year Final Research Report Summary
Increased fatty acid oxidation in immature reperfused hearts -Effects of insulin and glucagon-
| Project/Area Number |
11670777
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
ITOI Toshiyuki Kyoto Prefectural Universit of Medicine, Associate Professor, 医学部, 助教授 (10264780)
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| Project Period (FY) |
1999 – 2000
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| Keywords | immature heart / insulin / glucagon / fatty acid oxidation / reperfusion / malonyl-CoA |
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| Research Abstract |
Animal : Japanese white rabbit, two-week-old
Isolated working heart perfusion : 2O min normoxia-40 min ischemia-40 min reperfusion
Perfusion buffer : Krebs-Henselit (pH=7.4), including 1.25mM Ca^<2+>, 5.5mM glucose, 1.2mM ^<13>C-palmitate, 1.25mM lactate, 0.5mM acetoacetate.
Indication of cardiac function : heart rate x systolic pressure (double product, DP), cardiac output x systolic pressure (cardiac work, CW)
Myocardial ^<13>C-acetyl carnitine detection : Tandem mass
Myocardial CoA esters concentration : HPLC
Group : group 1 - no ischemia (40 min), insulin (40 U/ml) group 2 - no ischemia (40 min), glucagon (1 μg/ml) group 3 - reperfusion, insulin (40 U/ml) group 4 - reperfusion, glucagon (1 μg/ml)
RESULTS :
cardiac function (DP, CW) no ischemia : group 1 = group 2 reperfusion : group 3>group 4
palmitate oxidation no ischemia : group 1<group 2 reperfusion : group 3<group 4
tissue malonyl-CoA no ischemia : group 1>group 2 reperfusion : group 3>group 4
In reperfused immature hearts, glucagon may have detrimental effects on the recovery of contracrail function due to accelerate fatty acid oxidation.
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