2002 Fiscal Year Final Research Report Summary
Cytological characteristics of cord blood stem cells
| Project/Area Number |
11670782
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Dokkyo University School of Medicine |
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Principal Investigator |
EGUCHI Mitsuoki Dokkyo University School of Medicine, Professor, 医学部, 教授 (60020799)
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| Co-Investigator(Kenkyū-buntansha) |
MIKAMI Testuya Dokkyo University School of Medicine, Assistant Professor, 医学部, 講師 (60316586)
KUROSAWA Hidemitsu Dokkyo University School of Medicine, Assistant Professor, 医学部, 講師 (10205239)
SUGITA Kenichi Dokkyo University School of Medicine, Associate Professor, 医学部, 助教授 (80142404)
SATO Yuya Dokkyo University School of Medicine, Instructor, 医学部, 助手 (90265306)
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| Project Period (FY) |
1999 – 2002
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| Keywords | cord blood / stem cell / electron microscopy / leukocyte / cytokine / T lymphocyte / NK cell |
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| Research Abstract |
To identify the characteristics of cord blood cells, we examined their morphological features by electron microscopy and CXCR3 expression on CD4^+ cells and NK cells.
Compared with normal peripheral blood cells, cord blood cells frequently showed immature morphology and a unique ultrastructure, such as nuclear pockets in neutrophilis, several crystaloids in a single eosinophilic granulocytes and deformed nuclei in lymphocytes. In contrast to bone marrow cells, cord blood cells yielded a large number of cells of immature myelo-monocytic lineages in cell culture, and demonstrated weaker peroxidase reaction.
The percentage of CXCR3 antigen on cord blood and peripheral blood CD4^+ cells was 2.6% and 30.8%, respectively, demonstrating a significant difference. However, that on cord blood and peripheral blood NL cells was not significantly different suggesting milder acute graft-versus-host disease on cord blood stem cell transplantation.
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