2001 Fiscal Year Final Research Report Summary
Molecular biological approach for etiology of Kawasaki disease
| Project/Area Number |
11670799
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Nihon University |
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Principal Investigator |
HARADA Kensuke Nihon University School of Medicine, Department of Pediatrics, Professor, 医学部, 教授 (40208674)
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| Co-Investigator(Kenkyū-buntansha) |
AYUSAWA Mamoru Nihon University School of Medicine, Department of Pediatrics, 医学部, 助手 (40287610)
NOTO Nobutaka Nihon University School of Medicine, Department of Pediatrics, 医学部, 講師 (70267053)
OKADA Tomoo Nihon University School of Medicine, Department of Pediatrics, Associate Professor, 医学部, 助教授 (50177052)
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| Project Period (FY) |
1999 – 2001
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| Keywords | Kawasaki disease / IL-4 / RDA / IL-4STAT / Aminopeptidase N |
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| Research Abstract |
Representational difference analysis (RDA) was used to identify genes differentially expressed in the host genes of Kawasaki disease (KD). Two members of the interleukin-4 (IL-4) signal cascade were identified as a result. One was IL-4STAT and the other was aminopeptidase N (APN). In addition to the mRNA levels of IL-4STAT and APN, those of IL-4 and IL-4 receptor (IL-4R) in peripheral blood lymphocytes at the acute (KSA) and convalescent (KSC) phase in 12 KD patients and 5 non-KD febrile controls (FC) were determined by quantitative RT-PCR. The APN and IL-4STAT mRNA levels were significantly higher in KSA than in KSC or FC, and were not significantly different between KSC and FC. The IL-4 mRNA level was significantly lower in KSA than in KSC, and did not differ from that of FC. The IL-4R mRNA in KSA was significantly higher than that in FC, but not that in KSC. The APN, IL-4STAT and IL-4 mRNA levels of a patient with coronary artery aneurysm (CAn) did not differ from those of other patients without CAn. During the course of KD, significantly higher levels of mRNA than those in ordinary febrile disease were observed in the IL-4 cascade. This could be related specifically to the evocation and resolution of vasculitis in KD.
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