2000 Fiscal Year Final Research Report Summary
Longitudinal analysis of epitopes of anti-centromere antibodies in human and mouse
| Project/Area Number |
11670823
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | NAGOYA UNIVERSITY |
|---|
Principal Investigator |
MURO Yoshinao SCHOOL OF MEDICINE, NAGOYA UNIVERSITY, ASSOCIATE PROFESSOR, 医学部, 助教授 (80270990)
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Keywords | anti-centromere antibody / CENP-A / CENP-B / CENP-C / antigen-driven / autoantibody / autoantigen |
|---|
| Research Abstract |
I tried to analyze human anti-centromere antibodies (ACA) longitudinally and to produce animal antibodies, which mimic human ACA concerning on autoepitopes, experimentally. In human patients, there were various differences of autoimmune phenomenon against between CENP-B and CENP-A/C.Although rabbits immunized with synthetic peptides, which corresponded to human CENP-A autoepitopes, produced anti-CENP-A antibodies which could be used for both immunoblotting and immunofluorescence studies, they could not produce anti-CENP-B or anti-CENP-C antibodies under the observation of more than six months. I am now considering a few candidates of immunogen to pruduce experimental antibodies mimicing human ACA.
|
-
-
-
-
-
-
-
-
-
-
[Publications] Muro, Y., Azuma, N., Onouchi, H., Kunimatsu, M., Tomita, Y., Sasaki, M, Sugimoto, K.: "Autoepitopes on autoantigen CENP-A are restricted to the N-terminal region, which has no homology with histone H3."Clin.Exp.Immunol.. 120. 218-223 (2000)
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
