2000 Fiscal Year Final Research Report Summary
Molecular studies on the regulation of parathyroid cell growth and the mechanisms of parathyroid adenoma development
| Project/Area Number |
11671095
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Endocrinology
|
|---|
| Research Institution | Wakayama Medical University |
|---|
Principal Investigator |
SAKAGUCHI Kazushige Wakayama Medical University Professor, 付置研究所, 教授 (60178548)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YOKOTE Hideyuki Wakayama Med Univ, Mol Med, Assistant Prof, 付置研究所, 助手 (60316122)
NAKAMURA Masayuki Wakayama Med Univ, Mol Med, Assistant Prof, 付置研究所, 助手 (40145917)
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Keywords | parathyroid / tumor / oncogene / cDNA cloning / keratinocyte growth factor receptor / Yeast Two Hybrid / signal transduction |
|---|
| Research Abstract |
We have attempted systematical expression cloning of oncogenes from a cDNA library derived from a parathyroid adenoma, and functional analysis of these genes. An isoform of keratinocyte growth factor receptor, which has an alternatively spliced form of C-terminus, was isolated, and found to be expressed in parathyroid adenoma, but not in parathyroid cancer or hyperplasia. Two possibilities, the variant C terminus having functions of activating the signal transduction or the wild-type C terminus carrying the functions of inhibiting the signaling. To clarify these possibilities, we used the Yeast Two Hybrid system to clone the molecules that can bind to these C terminus portion of the keratinocyte growth factor receptor, which leads to the discovery of several molecules. We are now in the middle of characterizing the function of these clones.
|
