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2000 Fiscal Year Final Research Report Summary

The mechanisms of insulin-regulated exocytosis of the GLUT4-containing vesicles

Research Project

Project/Area Number 11671107
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionGunma University, Institute For Molecular and Cellular Regulation

Principal Investigator

SHIBATA Hiroshi  Institute for Molecular and Cellular Regulation, , Gunma University, Department of Cell Biology, Associate Professor, 生体調節研究所, 助教授 (20235584)

Project Period (FY) 1999 – 2000
Keywordsinsulin / glucose transporter / GTP-binding protein / Rab4 / syntaxin4 / SNARE
Research Abstract

In the present study, we investigated the interaction of Rab4 with syntaxin4, a t-SNARE protein implicated in the insulin-induced exocytic fusion of the GLUT4-containing vesicle with the plasma membrane. Rab4 and syntaxin4 were coimmunoprecipitated from the lysates of rat adipocytes. The interaction of the two proteins were attenuated by pretreatment of the cells with insulin but enhanced with GTPgS.A GTPase deficient mutant of Rab4, but not a GTP-binding defective mutant was bound to syntaxin 4, suggesting that the interaction between the two proteins were regulated by the guanine-nucleotide-binding state of Rab4. In addition, we found that the presence of munc-18c, a negative regulator of the SNARE comple formation, displaced Rab4 from syntaxin 4, indicating that the dissociation of munc-18c from syntaxin4 is required for Rab4 to bind to syntaxin 4.
We also clarified that the actin filaments play a critical role in exocytotic recruitment but not in endocytosis of GLUT4 in isolated rat adipocytes.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Omata,W. et al: "Actin filaments play a critical role in insulin-induced exocytotic recruitment but not in endocytosis of GLUT4 in isolated rat adipocytes."Biochem.J.. 346. 321-328 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 柴田宏: "インスリンシグナリングにおけるRabタンパク質"生体の科学. 50・6. 565-573 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanzaki,M. et al.: "Translocation of a calcium-permeable cation channel induced by insulin-like growth factor-I."Nature Cell Biol.. 1・3. 165-170 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohnishi,H. et al.: "Involvement of Rab4 in regulated exocytosis of rat pancreatic acini."Gastroenterology. 116. 943-952 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Li.L. et al.: "Direct interaction of Rab4 with syntaxin 4"J.Biol.Chem.. 276・7. 5265-5273 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,J. et al.: "Dynamin is involved in human epithelial cell vacuolation caused by the Helicobacter pylori-produced cytotoxin VacA"J.Clin.Invest.. 107・3. 363-370 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Omata, W., Shibata, H., Li, L., Takata, K.and Kojima, I.: "Actin filaments plays a critical role in insulin-induced exocytotic recruitment but not in endocytosis of GLUT4 in isolated rat adipocytes."Biochem.J.. 346. 321-328 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li, L., Omata, W., Kojima, I.and Shibata, H.: "Direct interaction of Rab4 with syntaxin 4."J.Biol.Chem.. 276. 5265-5273 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, J., Ohnishi, H., Shibata, H., Iiri, T., Wada, A., Hirayama, T., Ueda, N., Kanamatsu, C., Tsuchida, T., Mashima, H., Yasuda, H.and Fujita, T.: "Dynamin is involved jin the vacuolation induced by Helicobacter Pylori vacuolating cytotoxin (VacA)."J.Clin.Invest.. 107. 363-370 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shibata, H.: "Insuiln signaling and Rab."Seitai no Kagaku. 50. 565-573 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanzaki, M., Zhang, Y.-Q., Mashima, H., Li, L., Shibata, H.and Kojima, I.: "Transloacation of a calcium-permeable channel induced by insulin-like growth factor-I."Nature Cell Biol.. 1. 165-170 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohnishi, H., Mine, T., Shibata, H., Ueda, N., Tsuchida, T., and Fujita, T.: "Involvement of Rab4 in regulated exocytosis of rat pancreatic acini."Gastroenterology. 116. 943-952 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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