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2000 Fiscal Year Final Research Report Summary

THE TARGET GENE FOR PPARα AND PPARγ IN HUVEC

Research Project

Project/Area Number 11671135
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionSaitama Medical School

Principal Investigator

INOUE Ikuo  Saitama Medical School,, 医学部, 講師 (60232526)

Project Period (FY) 1999 – 2000
Keywordsperoxisome proliferator-activated receptor α (PPARα) / peroxisome proliferator-activated receptor γ (PPARγ) / human umbilical vein endothelial cells (HUVEC) / nicotine adenine dinucleotide phosphate (NADPH) oxidase / cyclooxygenase-2 / interleukin-1β / Cu2+, Zn2+-superoxide dismutase (CuZn-SOD)
Research Abstract

We examined the effects of peroxisome proliferator-activated receptor α (PPARα) and PPARγ on the production and expression of inflammatory cytokines and on enzyme expression involving prostaglandin and superoxide production in cultured human umbilical vein endothelial cells (HUVEC). PPARα and PPARγ significantly reduced interleukin-1β and -6 mRNA expression and their protein levels in the culture medium, and also inhibited cyclooxygenase-2 mRNA expression and their protein levels. Moreover, the mRNA levels of p22phox, a 22-kD subunit and the protein levels of p47phox, a 47-kD subunit of nicotine adenine dinucleotide phosphate (NADPH) oxidase, was decreased by induction of PPARα and PPARγ. This unique anti-inflammatory effect in addition to its hypolipidemic action, may be beneficial in preventung the vascular complications that are induced by hyperlipidemia.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Inoue I, et al.: "The ligands/activators for peroxisome proliferator-activated receptor alpha (PPARalpha) and PPARgamma increase Cu2+, Zn2+-superoxide dismutase and decrease p22phox message expressions in primary endothelial cells."Metabolism. 50(1). 3-11 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inoue I,Katayama S: "Hypertension and reactive oxygen species"Nippon Rinsho. 58(Sup1). 193-9 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inoue I: "Molecular biology of lipid metabolism in vascular wall."Nippon Rinsho. 59(Sup2). 580-585 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inoue I, et al: "Lipophllic HMG-CoA reductase inhibitor has an anti-inflammatory effect : reduction of MRNA levels for interleukin-1beta, interleukin-6, cyclooxygenase-2, and p22phox by regulation of peroxisome proliferator-activated receptor alpha (PPARalpha) in primary endothelial cells."Life Sci. 67(8). 863-76 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Awata T,Inoue K,Inoue I: "Lack of association of the Ala45Thr variant in the BETA2/NEUROD1 with type 1 diabetes in Japanese."Diabetes Res Clin Pract. 49(1). 61-3 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 井上郁夫: "血管内皮細胞におけるPPARαの発現と動脈硬化のMultiple Risk Factorとの関連性"動脈硬化. 28(3). 55-62 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inoue I, Goto S, Noji S, et al: "(5) The ligands/activators for peroxisome proliferator-activated receptor a (PPARa) and PPARg increase Cu2+, Zn2+-superoxide dismutase (CuZn-SOD) and decrease p22phox message expressions in primary endothelial cells."Metabolism. 50. 3-11 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inoue I, Goto S, Mizotani K, et al: "(7) Lipophilic HMG-CoA reductase inhibitoror has an anti-inflammatory effect : reduction of mRNA levels for interleukin-1b, interleukin-6, cyclooxygenase-2, and p22phox br regulation of reroxisome proliferator-activated receptor a in primary endothelial cells."Life Science. 67. 863-876 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakajima T, Origuchi N, Matsunaga T, Kawai S, Hokari S, Nakamura H, Inoue I, et al.: "(3) Location of oxidized HDL in atheromatous plaques and oxidized HDL binding site on human aortic endothelial cells."Ann Clin Biochem. 37 (Pt2). 179-86 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Awata T, Inoue K, Inoue I, et al.: "(4) Lack of association of the Ala45Thr variant in the BETA2/NEUROD1 with type 1 diabetes in Japanese."Diabetes Res Clin Pract. 49. 61-63 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Awata T, Inoue K, Kurihara S, Ohkubo T, Inoue I, et al.: "(4) Missense Variations of the Gene Responsible for Wolfram Syndrome (WFS1/wolframin) in Japanese : Possible Contribution of the Arg456His Mutation to Type 1 Diabetes as a Nonautoimmune Genetic Basis."Biochem Biophys Res Commun. 268. 612-616 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsunaga T, Nakajima T, Sonodaq M, Kawai S, Kobayashi J, Inoue I, Katayama S, et al.: "(5) Reactive oxygen species as a risk factor in verotoxin 1-exposed rats."Biochem Biophys Res Commum. 14 : 260. 813-819 (1999)

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      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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