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2000 Fiscal Year Final Research Report Summary

Establishment of the anti-tumor immunotherapy using dendritic cells transfected with tumor extracted RNA

Research Project

Project/Area Number 11671152
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionThe University of Tokyo

Principal Investigator

OSADA Takuya  Univ. of Tokyo Hospital/Assistant, 医学部・附属病院, 助手 (90272559)

Co-Investigator(Kenkyū-buntansha) KITAYAMA Joji  Univ. of Tokyo Hospital/lecturer, 医学部・附属病院, 講師 (20251308)
TAKAMIZAWA Masaru  Univ. of Tokyo Hospital/lecturer, 医学部・附属病院, 講師 (40216784)
SHIBATA Yoichi  Univ. of Tokyo Hospital/Prof., 医学部・附属病院, 教授 (30010474)
TSUNO Nelson  Univ. of Tokyo Hospital/Assistant, 医学部・附属病院, 助手 (50282637)
Project Period (FY) 1999 – 2000
Keywordsdendritic cell / immunotherapy / cytotoxic T lymphocyte / colon cancer / RNA / tumor antigen
Research Abstract

In the present examination, it was possible to establish a cytotoxic T lymphocyte by introducing mRNA extracted from human colon cancer cell line into the human dendritic cell, and coculturing with T lymphocyte in in vitro. It was shown that this CTL selectively killed only the cell line from which the introduced RNA was extracted. And, the induction efficiency of CTL, when it was compared in the case in which Liposome was used and case in which it was not used, though it was evaluated of Liposome, as it is efficient a little, no significant difference could be observed. Though the efficiency of the mRNA recovery from the human surgery specimen was examined, the yield point was greatly influenced in fixation situation of samples as estimated, and mRNA was also generally fragmented. Therefore, it seemed not to be effective to introduce RNA extracted from these fixed specimen into dendritic cells. On the contrary, freezing samples seemed to be suitable as a material for this method, since mRNA was abounding on the yield point, and since the fragmentation was also little. Based on the present examination, it was shown that by extracting mRNA not only from large specimen of surgical sample but also from small samples such as the biopsy piece, it is possible to make dendritic cell vaccine which presented the tumor antigens specific to the patient's tumor. These results indicate further adaptation extension of the dendritic cell therapy.

  • Research Products

    (48 results)

All Other

All Publications (48 results)

  • [Publications] Osada T, et al.: "Thoracic empyema associated with recurrent colon cancer: report of a case and review of the literature"Piseases of Colon & Rectum. 44. 291-294 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osada et al.: "Prognostic significance of glutamine synthetase expression in unifocal advanced hepatocellular carcinoma"Journal of Hepatology. 33. 247-253 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihara S, Osada T, et al: "α-glycosylceramides enhance the antitumor cytotoxicity of hepatic lymphocytes obtained from cancer patients by activating CD3-CD56+ NK cells in vitro"J. Immunol. 165. 1659-1664 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sunami E, Osada T, et al: "MMP-1 is a prognostic marker for hematogenous metastasis of colorectal cancer."Oncologint. 2000. 114 (108)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitayama J, Osada T, et al: "E-selectin can mediate the arrest type of adhesion of colon cancer cells under physiological shear flow"European Joural of Caucer. 36. 121-127 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito S, Osada T, et al: "Expression of platelet-derived endothelial cell growih faetor correlats with good prograsic inpatients with colorutal caroinars"Caucer. 88. 42-49 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osada T, Sakamoto M, et al.: "Acquisition of glutamine synthetase expression in human hepatocarcinogenesis."Cancer. 85. 819-31 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomozawa S, Osada T, et al.: "Cyclooxigenase-2 overexpression correlates with tumour recurrence especially haematogeneous metastasis, of colorectal cancer."Br J Cancer. 83. 324-8 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomozawa S, Osada T, et al.: "Inhibition of haematogenous metastasis of colon cancer in mice by a selective COX-2 inhibitor, JTE-522."Br J Cancer. 81. 1274-9 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitayama J, Osada T, et al.: "Laminin mediate tethering and spreading of colon cancer cells in physiological shear flow."Br J Cancer. 80. 1927-34 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihara S, Osada T, et al.: "CD8(+)NKR-P1A(+)T cells preferentially accumulate in human liver. Eur J"Immunol. 29. 2406-13 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagashima I, Osada T, et al.: "Histopathologic prognostic factors influencing long-term prognosis after surgical resection for hepatic metastases from colorectal cancer."Am J Gastro enterol. 94. 739-43 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nita ME, Tsuno N, et al.: "Bcl-X(L) antisense sensitizes human colon cancer cell line to 5-fluorouracil. Jpn"J Cancer Res. 91. 825-32 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nita ME, Tsuno N, et al.: "p21 Wafl/Cipl expression is a prognostic marker in curative resected esophageal squamous cell carcinoma, but not p27Kipl, p53, or Rb."Ann Surg Oncol. 6. 481-8 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsurita G, Tsuno NH, et al.: "Effects of exposure to repetitive pulsed magnetic stimulation on cell proliferation and expression of heat shock protein 70 in normal and malignant cells. "Biochem Biophys Res Commun. 261. 689-94 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sameshima S, Tsuno N, et al.: "Correlation of the therapeutic effect of activated tumor-draining lymphnode cells with specific interferon-gamma production in vitro."Surg Today. 29. 55-62 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayama H, Kitayama J, et al.: "Characterization of intracellular cytokine profile of CD4(+)T cells in peripheral blood and tumor-draining lymphnodes of patients with gastrointestinal cancer."Jpn J Clin Oncol. 30. 301-5 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitayama J, Hidemura A, et al.: "Shear stress affects migration behavior of polymorphonuclear cells arrested on endothelium."Cell Immunol. 203. 39-46 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takenoue T, Kitayama J, et al.: "CHaracterization of dihydropyrimidine dehydrogenase on immunohistochemistry in colon carcinoma, and correlation between immunohistochemical score and pretein level or messenger RNA expression."Ann Oncol. 11. 273-9 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takenoue T, Kitayama J, et al.: "Relation between thymidylate synthase expression and survival in colon carcinoma, and determination of appropriate application of 5-fluorouracil by immunohistochemical method."Ann Sug Oncol. 7. 193-8 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuda K, Kitayama J, et al.: "Clinical significance of MUC1 and MUC2 mucin and p53 protein expression in colorectal carcinoma."Jpn J Clin Oncol. 30. 89-94 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitayama J, Ikeda S, et al.: "Alpha 6 beta 1 integrin (VLA-6) mediates lenkocyte tether and arrest on laminin under physiological shear flow."Cell Immunol. 199. 97-103 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seto Y, Kitayama J, et al.: "Exposure and dissection of the root of the right gastric artery during gastric surgery."Surg Today. 29. 1229-30 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitayama J, Tuno N, et al.: "Functional down-regulation of betal and beta2 integrins of lamina propria lymphocytes (LPL) and tumor-infiltating lymphocytes (TIL) in colorectal cancer patients."Ann Surg Oncol. 6. 500-6 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osaka T, Nagawa H, et al.: "Thoracic empyema associated with recurrent colon cancer : report of a case and review of the literature."Dis Colon Rectum. 44. 291-4 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Osada T, Nagashima I, et al.: "Prognostic significance of glutamine synthetase expression in unifocal advanced hepatocellular carcinoma."J Hepatol. 33. 247-53 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Osada T, Sakamoto M, et al.: "Acquisition of glutamine synthetase expression in human hepatocarcinogenesis."Cancer. 85. 819-31 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomozawa S, Osada T, et al.: "Cyclooxigenase-2 overexpression correlates with tumour recurrence especially haematogeneous metastasis, of colorectal cancer."Br J Cancer. 83. 324-8 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihara S, Osada T, et al.: "Alpha-glycosylceramides enhance the antitumor cytotoxicity of hepatic lymphocyte obtained from cancer patients by activating CD3-CD56+NK cells in vitro."J immunol. 165. 1659-64 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sunami E, Osada T, et al.: "MMP-1 is a prognostic marker for haematogenous metastasis of colorectal cancer."Oncologist. 5. 108-14 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitayama J, Osada T, et al.: "E-selectin can mediate the arrest type of adhesion of colon cancer cells under physiological shear flow."Eur J Cancer. 36. 121-7 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito S, Osada T, et al.: "Expression of platelet-derived endothelial cell growth factor correlate with good prognosis in patients with colorectal carcinoma."Cancer. 88. 42-9 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomozawa S, Osada T, et al.: "Inhibition of haematogenous metastasis of colon cancer in mice by a selective COX-2 inhibitor, JTE-522."Br J Cancer. 81. 1274-9 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitayama J, Osada T, et al.: "Lamiuin mediate tethering and spreading of colon cancer cells in Physiological shear flow."Br J Cancer. 80. 1927-34 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihara S, Osada T, et al.: "CD8(+)NKR-P1A(+) T cells preferentially accumulate in human liver."Eur J Immunol. 29. 2406-13 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagashima I, Osada T, et al.: "Histopathologic prognostic factors influencing long-term prognosis after surgical resection for hepatic metastases from colorectal cancer."Am J Gastro enterol. 94. 739-43 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nita ME, Tsuno N, et al.: "Bcl-X(L) antisense sensitizes human colon cancer cell line to 5-fluorouracil"Jpn J Cancer Res. 91. 825-32 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nita ME, Tsuno N, et al.: "p21 Waf1/Cip1 expression is a prognostic marker in curative resected esophageal squamous cell carcinoma, but not p274Kip1, p53, or Rb."Ann Surg Oncol. 6. 481-8 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsurita G, Tsuno NH, et al.: "Effects of exposure to repetitive pulsed magnetic stimulation on cell proliferation and expression of heat shock protein 70 in normal and malignant cells."Biochem Biophys Res Commun. 261. 689-94 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sameshima S, Tsuno N, et al.: "Correlation of the therapeutic effect of activated tumor-draining lymphnode cells with specific interferon-gamma production in vitro."Surg Today. 29. 55-62 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayama H, Kitayama J, et al.: "Characterization of intracellular cytokine profile of CD4(+)T cells in peripheral blood and tumor-draining lymphnodes of patients with gastrointestinal cancer."Jpn J Clin Oncol. 30. 301-5 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitayama J, Hidemura A, et al.: "Shear stress affects migration behavior of polymorphonuclear cells arrested on endothelium."Cell Immunol. 203. 39-46 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takenoue T, Kitayama J, et al.: "CHaracterization of dihydropyrimidine dehydrogenase on immunohistochemistry in colon carcinoma, and correlation between immunohistochemical score and pretein level or messenger RNA expression."Ann Oncol. 11. 273-9 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takenoue T, Kitayama J, et al.: "Relation between thymidylate synthase expression and survival in colon carcinoma, and determination of appropriate application of 5-fluorouracil by immunohistochemical method."Ann Sug Oncol. 7. 193-8 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuda K, Kitayama J, et al.: "Clinical significance of MUC1 and MUC2 mucin and p53 protein expression in colorectal carcinoma."Jpn J Clin Oncol. 30. 89-94 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitayama J, Ikeda S, et al.: "Alpha 6 beta 1 integrin (VLA-6) mediates leukocyte tether and arrest on laminin under physiological shear flow."Cell Immunol. 199. 97-103 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Seto Y, Kitayama J, et al.: "Exposure and dissection of the root of the right gastric artery during gastric surgery."Surg Today. 29. 1229-30 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitayama J, Tuno N, et al.: "Functional down-regulation of beta1 and beta2 integrins of lamina propria lymphocytes (LPL) and tumor-infiltating lymphocytes (TIL) in colorectal cancer patients."Ann Surg Oncol. 6. 500-6 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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