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2001 Fiscal Year Final Research Report Summary

Alloimmunization and immunomodulation induced by blood transfusion Analysis by microchimerism.

Research Project

Project/Area Number 11671173
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionFukushima Medical University

Principal Investigator

OHTO Hitoshi  Fukushima Medical University School of Medicine, Professor, 医学部, 教授 (20150279)

Co-Investigator(Kenkyū-buntansha) SHIKAMA Yayoi  Fukushima Medical University School of Medicine, Assistant, 医学部, 助手 (40291562)
Project Period (FY) 1999 – 2001
Keywordsalloimmunization / microchimerism / tolerance / blood transfusion surgical patients / pregnancy leukoreduction / newborn / surgical patients / leukore douction
Research Abstract

Background : Donor-derived microchimerism may be involved with transfusion-related immunomodulation. Immunization to an allogeneic antigen can occur exclusively against tolerance, in the latter microchimerism may play a key role. Methods: 1. Surgical recipients : To confirm that the use of leukoreduction filters efficiently prevent alloimmunization against HLA, 47 patients u sing a leukoreduction filter and 40 patients using an aggregate-removing filter were studied. 2. Pregnant women : By amplifying Y chromosome DNA in peripheral blood of 20 male-pregnant women, fetus-derived microchimerism was studied. 3. Infants transfused : 30 children with blood transfusion history 5-ll years ago were tested for the sensitization against HLA alloantigens. Results : 1. Surgical recipients : The alloimmunization rate of recipients (17%) with a leukoreduction filter did not differ from the recipients (5%) using an aggregate filter, nevertheless the former patients were transfused much more number of … More leukocytes (1234x10^6) than the latter recipients (0.3x10^6). 2. Pregnant women : All male-pregnant women had fetus-derived Y chromosome DNA since around 7-16 weeks of gestation, and the DNA gradually increased to parturition followed by a rapid disappearance after parturition. 3. Infants transfused : No infants were positive for anti-HLA allantibody but 2 of 28 mothers had anti-HLA reactive with their infants and their husbands (fathers). Discussion : Surgical recipients are intact in allorecognition through indirect pathway. And thus, surgical recipients using a high efficiency leukoreduction filter acquire allo-HLA antibody equivalently to patients receiving with an aggregate filter. Newborn infants, who are imp aired in T cell functions, cannot amount alloimmunization against HLA even after many leukocytes are transfused. All pregnant women develop microchimerism during pregnancy and parturition. Further stud y is needed to determine alloantigen-tolerance is closely related to the establishment of permanent micro chimerism of the same antigen-positive infants. Less

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Ohto Hitoshi: "HLA antibodies after transfusion of FFP"Transfusion. 40(5). 613-614 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ariga Hiromichi, Ohto Hitoshi, et al.: "Kinetics of fetal cellular and cell-free DNA in the maternal circulation during and after pregnancy : implication for noninvasive prenatal diagnosis"Transfusion. 41(12). 1524-1530 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安田広康, 大戸 斉, 他: "白血球除去フィルター使用輸血における同種免疫感作の頻度と危険因子"日本輸血学会雑誌. 46(1). 7-11 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤森敬也, 大戸 斉, 他: "経胎盤胎児由来細胞母体血への移行"産婦人科の世界. 52(10). 831-834 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 榎本孝行, 大戸 斉, 他: "妊娠による血小板反応性抗体(HLA抗体とhuman platelet antigens抗体の産生:妊娠回数及び週齢と抗体陽性率の解析"日本輸血学会雑誌. 46(5). 467-473 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 今村真哉, 大戸 斉, 他: "妊娠によるMicrochimerismの成立"福島医学雑誌. 51(2). 113-119 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大戸 斉, 他: "輸血医学における分子生物学 第7章 輸血と妊娠・分娩によるマイクロキメリズム"池田久実. 7 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohto H: "HLA antibodies after transfusion of FFP"Transfusion. 40(5). 613-614 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ariga H, Ohto H, et al: "Kinetics of fetal cellular and cell-free DNA in the maternal circulation during and after pregnancy : implication for non invasive prenatal diagnosis"Transfusion. 41(12). 1524-1530 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohto H, et al: "Alloimmunization by transfusion with bedside leukoreduction filters in immunocompetent recipients: a prospective study"Transfusion. 40. 11s (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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