2000 Fiscal Year Final Research Report Summary
Overexpression of G2/M cyclin in patients with gastrointestinal cancer. An immunohistological study
Project/Area Number |
11671198
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General surgery
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Research Institution | Fukuoka Dental College |
Principal Investigator |
HONDA Masayuki Fukuoka Dental College, Dentistry, Assistant, 歯学部, 助手 (40330972)
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Co-Investigator(Kenkyū-buntansha) |
YASUDA Mitsuhiro Fukuoka Dental College, Dentistry, Assistant, 歯学部, 助手 (90269043)
INUTSUKA Sadaaki Fukuoka Dental College, Dentistry, Lecturer, 歯学部, 講師 (40258596)
KORENAGA Daisuke Fukuoka Dental College, Dentistry, Professor, 歯学部, 教授 (90170414)
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Project Period (FY) |
1999 – 2000
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Keywords | cell cycle / cyclin / lymphnode metastasis / G2 / M checkpoint / gastric cancer / colorectal cancer / 細胞増殖活性 |
Research Abstract |
PURPOSE : This study was done to determine if overexpression of G2/M cyclin is related to tumor invasion and its metastatic potential. METHODS : The expressions of Cyclin B (C-B) were immunohistologically determined in 109 patients with advanced colorectal cancer (Dukes B, C, D) and in 61 patients with gastric cancer. The data were compared with findings obtained by Ki-67, PCNA and p53 immunohistological stainings. RESULTS : C-B was expressed in 62 (57%) patients with colorectal cancer and in 32 (53%) of those with gastric cancer. In cases with colorectal cancer, the incidence cf Dukes B tumors in the C-B positive cases was significantly higher than that in the C-B negative cases (p<0.05). The incidence of lymph node metastasis in the C-B positive cases was significantly lower than that in C-B negative cases (p<0.05). Similar tendency was observed with regard to lymphatic permeation and venous invasion (p<0.05). There were no correlations of C-B negative tumors with higher proliferative activities determined by Ki-67, PCNA and p53 analyses. In the logistic regression analysis, only lymphatic permeation proved to be significant risk factors relating to lymph node metastasis. In those with gastric cancer, the C-B negative cases were seen in younger patients, in those with poorly differentiated adenocarcinoma, in tumors with infiltrative growth type (p<0.05). Advanced stage of the disease (stages III and IV) and widespread nodal involvement were frequent in the C-B negative cases compared with C-B positive cases (p<0.05). Multivariate analysis showed that histological type significantly correlated with C-B expression. CONCLUSIONS : These findings suggested that the C-B negative tumors might have a higher tendency for tumor invasion and metastasizing to lymph node, and the potential role of C-B overexpression on the metastatic formation was indicated.
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