2002 Fiscal Year Final Research Report Summary
Expression and function of cyclooxygenase-2 in human colorectal adenomas and cancers
| Project/Area Number |
11671212
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Tokyo Medical & Dental University |
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Principal Investigator |
ENOMOTO Masayuki Tokyo Medical and Dental University Graduate School, Department of Digestive Surgery, Assistant Professor, 大学院・医歯学総合研究科, 講師 (60301165)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIHARA Kenichi Tokyo Medical and Dental University Graduate School, Department of Digestive Surgery, Professor, 大学院・医歯学総合研究科, 教授 (10171167)
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| Project Period (FY) |
1999 – 2002
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| Keywords | Cycooxygenase-2 / COX-2 / colorectal adenoma / colorectal cancer |
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| Research Abstract |
Cyclooxygenase-2 (COX-2) is an important target for the suppression of colorectal tumorigenesis. To evaluate the role of COX-2 in human adenomas and cancers of sporadic and familial adenomatous polyposis., COX-2 expression was examined by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). All adenomas shows COX―2 mRNA expression and COX―2 indices (Cox2 m-RNA / Coxl m-RNA) in the adenomas were significantly higher than that in normal mucosa(P<0.0001).Positive correlation was found between the COX―2 indices and the size of adenomas by simple regression and Spearman's coefficient by rank ( R=0.34, P=0.007). Immunohistochemistry showed that the expression of COX-2 protein was significantly correlated with the degree of displasia and 72.6 percents of COX-2 protein was located in the cytoplasm. In human cancers, COX-2 indices in normal mucosa, polypoid cancer and non-polypoid cancer were 0.7 ±0.1, 1.0 ±0.1 and 1.9 ± 0.1, respectively. The COX-2 index in non-polypoid cancer was significantly higher than in polypoid cancer (P=0.0002) or normal mucosa(P<0.0001)
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