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2000 Fiscal Year Final Research Report Summary

Expression of Aquaporins in Ileal W-type reservoir

Research Project

Project/Area Number 11671214
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

SUDA Takeyasu  School of Medicine, NIIGATA UNIVERSITY, Lecturer, 医学部, 講師 (50251806)

Co-Investigator(Kenkyū-buntansha) IIAI Tsuneo  University Medical Hospital, NIIGATA UNIVERSITY, Assistant, 医学部・附属病院, 助手 (10323982)
SATOH Nobuaki  University Medical Hospital, NIIGATA UNIVERSITY, Associate Professor, 医学部・附属病院, 助教授 (90215822)
OKAMOTO Haruhiko  University Medical Hospital, NIIGATA UNIVERSITY, Assistant, 医学部・附属病院, 助手 (90301184)
YAMAMOTO Tadashi  School of Medicine, NIIGATA UNIVERSITY, Professor, 医学部, 教授 (30092737)
Project Period (FY) 1999 – 2000
Keywordswater channel / AQP8 / AQP9 / colon / subtotal colectomy / ulcerative colitis / ileal W-pouch / colonization
Research Abstract

<STUDY 1>
Human aquaporin 8 (AQP8) cDNA was isolated from a human large intestine cDNA library by homology cloning. The open reading frame encoded 261 amino acid protein and the amino acid sequence was 75% identical to rat AQP8 and 76% identical to mouse AQP8. Expression of human AQP8 cRNA in Xenopus oocytes increased osmotic water permeability in a mercury-sensitive manner, indicating a water channel function of this molecule. Northern blot analysis and ribonuclease protection assay showed intense expression of 〜1.35-kilobase human AQP8 transcript in pancreas and colon, and weak expression in appendix and rectum. By in situ hybridization, AQP8 mRNA expression was localized in absorptive colonic epithelial cells, suggesting a role of AQP8 in the water absorption in the colon. The human AQP8 gene was mapped to 16p11.2-p12 by radiation hybrid mapping.
<STUDY 2>
[Background] Water has been presumed to move between epithelial cells (paracellular route) by interpretation of electrophysiological studies. But recently, water channel (AQP) mRNA espression was demonstrated in the gastrointestinal tract, and transcellular route of water for absorption and secretion was suggested.
[Purpose] Using a rat subtotal colectomy model, we investigated the role of AQP in the intestinal tract.
[Method] The effects of surgical resection on the expression of AQP mRNA were assesed by a RNase Protection Assay.
[Results] After subtotal colectomy, expression of rat AQP8 mRNA was markedly increased in the remnant colon.
[Discussion] Induction of AQP mRNA in remnant colon may contribute to the recovery from the increased water loss after subtotal colectomy.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Kouei Nibei: "Immunolocalization of Aquaporin-9 in Rat Hepatocytes and Leydig Cells."Arch.histol.Cytol.. 64. 81-88 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yu Koyama: "Molecular Cloning of a New Aquaporin from Rat Pancreas and Liver."J.Biol.Chem.. 272. 30329-30333 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yu Koyama: "Expression and Localization of Aquaporin in Rat Gastrointestinal tract."Am.J.Physiol.. 276. C621-627 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 須田武保: "潰瘍性大腸炎重症度とその外科治療"消化器外科. 23. 49-55 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 須田武保: "炎症性腸疾患の手術適応"臨床医. 26. 1668-1670 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Maruta Tomoaki: "Key Factors Influencing Bowel Function After Heal W-Pouch Anal Anastomosis : A Spectral Analysis of W-Pouch Motor Activity"Surgery Today. 30. 886-891 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kouei Nihei, Yu Koyama, Tatsuo Tani, Eishin Yaoita, Kazufumi Ohshiro, Laxman P.Adhikary, Isao Kurosaki, Yoshio Shirai, Katsuyoshi Hatakeyama, Tadashi Yamamoto.: "Immunolocalization of Aquaporin-9 in Rat Hepatocytes and Leydig Cells."Arch.Histol.Cytol.. 64. 81-88 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yu Koyama, Tadashi Yamamoto, Daisuke Kondo, Haruko Funaki, Eishin Yaoita, Katsutoshi Kawasaki, Nobuaki Sato, Katsuyoshi Hatakeyama, Itaru Kihara.: "Molecular Cloning of a New Aquaporin from Rat Pancreas and Liver."J.Biol.Chem.. 272. 30329-30333 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yu Koyama, Tadashi Yamamoto, Tatsuo Tani, Kouei Nihei, Daisuke Kondo, Haruko Funaki, Eishin Yaoita, Katsutoshi Kawasaki, Nobuaki Sato, Katsuyoshi Hatakeyama, Itaru Kihara.: "Expression and Localization of Aquaporin in rat gastrointestinal tract."Am.J.Physiol.. 276. C621-627 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeyasu Suda, Yasuo Sakai, Chieko Araki, Tsuneo Iiai, Kimitoshi Shimamura, Haruhiko Okamoto, Katsuyoshi Hatakeyama: "UC-Clinical Stage and Surgical Treatment."SHOUKKIGEKA. 23. 49-55 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeyasu Suda, Tsuneo Iiai, Haruhiko Okamoto, Katsuyoshi Hatakeyama.: "Surgical Indication in Inflammatory Bowel Disease."RINNSYOUI. 26. 1668-1670 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maruta Tomoaki, Honma Shinnji, Yagi Minoru, Hasegawa Jun, Shimamura Kimitoshi, Suda Takeyasu, Sakai Yasuo, Hatakeyama Katsuyoshi.: "Key Factors Influencing Bowel Function After Ileal W-Pouch Anal Anastomosis : A Spectral Analysis of W-Pouch Motor Activity."Sugery Today. 30. 886-891 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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