2000 Fiscal Year Final Research Report Summary
Development of severe myocardial infarction with gene engineered auto cardiomyoblast
| Project/Area Number |
11671303
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | YAMAGATA UNIVERSITY |
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Principal Investigator |
MINOWA Takashi Yamagata Univ, Med, Assistant Professor, 医学部, 助手 (50292420)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Takao Yamagata Univ, Med, Associate Professor, 医学部, 助教授 (60138922)
INUI Kiyoshige Yamagata Univ, Med, Assistant Professor, 医学部, 助手 (70250941)
SHIMAZAKI Yasuhisa Yamagata Univ, Med, Professor, 医学部, 教授 (60116043)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Gene therapy / Cell transplantation / VEGF / Myocardial infarction |
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| Research Abstract |
In this experiment, whether gene transfected cell transplanted in the organ can survive in the tissue and change the circumference organization was examined. Thoracotomy of 400g SD rat was carried out under the intratracheal intubation, and the left ventricular myocardium was cryoinjured by metallic tip cooled by liquid nitrogen. Two weeks after the injury of myocardium, thoracotomy through the same intercostal space was carried out, and 500000 VEGF cDNA transfected H9c2 cells in the 100 microlitter medium were injected in the injured myocardium. Two weeks after the cell transplantation, rats were sacrificed. Heart were fixed in the buffered formalin, paraffin embedded, sliced 5 micrometer thickness and stained by HE staining or immunohistochemical staining using anti-CD34 antibody or anti-6xHis antibody. Numerous capillaries in the damaged myocardium were recognized. And CD34 immunostaining showed that the cell in the capillaries were endothelium. Anti-6xHis immunostaining showed that the VEGF was excreted around the transplanted cells and the massive capillary formation was restricted in the positive staining area of VEGF.Our data showed that the secretory protein from the gene transfected cell transplanted by injection in the damaged myocardium could reorganize the surrounding tissue.
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