2000 Fiscal Year Final Research Report Summary
Research of angiogenic therapy of myocardial infarction with intramyocardial administration of basic fibroblast growth factor
| Project/Area Number |
11671311
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Thoracic surgery
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
KAWASUJI Michio Kanazawa Univ. School of Med. Associate Professor, 医学部, 助教授 (40135067)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YADUDA Tamotsu Kanazawa Univ. School of Med. Lecturer, 医学部・附属病院, 助手 (80324119)
FUJII Susumu Kanazawa Univ. School of Med. Lecturer
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Keywords | Myocardial infarction / Basic fibroblast growth factor / Angiogenesis |
|---|
| Research Abstract |
This study was designed to evaluate the effects of intramyocardial administration of basic faibroblast growth factor (bFGF) on myocardial blood flow, angiogenesis, and ventricular function in a canine acute myocardial infarction model. Myocardial infarction was induced in dogs by ligation of the left anterior descending coronary artery. Within 5 minutes after coronary occlusion, 100μg of bFGF in 1 mL of saline was injected into the infarct and border zone in 6 dogs, whereas saline alone was used in 6 control dogs. Myocardial blood flow was determined with colored microsphere before and immediately after coronary ligation and again 3, 7, 14, and 28 days after treatment. Angiogenesis was evaluated by immunohistochemical studies 28 days later. Treatment with bFGF significantly increased the endocardial blood flow in the border zone as well as epicardial blood flow in the infarcted zone. Treatment with bFGF significantly increased the capillary density as well as arteriolar density in the border zone. Treatment with bFGF significantly reduced the change in ratio of thickness of the infarcted wall to the normal wall. It improved the left ventricular ejection fraction. Intramyocardial administration of bFGF increased the regional myocradial blood flow, reduced thinning of the infracted region, and improved ventricular function. Intramyocardial adminiastration of bFGF may be a new therapeutic approach for patients with acute myocardial infarction.
|
