2001 Fiscal Year Final Research Report Summary
Prophylactic effect of proton pump inhibitor in the development of bronchiolitis obliterans after lung transplantation in rats
| Project/Area Number |
11671346
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
SAITO Yukihito Kansai Medical University,Medicine,Associate Professor, 医学部, 助教授 (50131438)
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| Co-Investigator(Kenkyū-buntansha) |
OMIYA Hideyasu Kansai Medical University, Medicine, Assistant Professor, 医学部, 助手 (70309219)
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| Project Period (FY) |
1999 – 2000
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| Keywords | lung transplantation / bronchiolitis obliterans / proton pump inhibitor |
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| Research Abstract |
Background and Objectives. Bronchiolitis obliterans is the most significant complication adversely affecting prolonged survival of lung allograft recipients. The evolution from the initial isult to the final pathologic entity is largely unknow. The aim of this study was to evaluate the efficacy of proton pump inhibitor in the development of bronchiolitis obliterans after lung transplantation in rats.
Methods(Experimental stydy 1) : Donor lung were transplanted from Brown Norway rats to Brown Norway rats (isografts ; group A) or to Lewis rats (allograft ; group B. Cyclosporine was administrated into rats of group B. Implanted lung in both groups were harvested at 3 different time points (from 30, 60 and 90 day after transplantation) and assessed immunohistochemical study using nitrotyrosin for detecting of bronchiolitis obliterans.
Methods(Experimental stydy 2) : Tracheal segments were transplanted from Brown Norway rats to Brown Norway rats (isografts ; group A) or to Lewis rats (allograft ; group B). Grafts were implanted into a subcutaneous pouch and an abdominal omental wrap. Proton pump inhibitor (200μ g/kg/day of bafilomycin A1) was administrated into rats of group B for 21days.
Results and Conclusions : Exp. 1 : Immunohistochemical staining using nitrotyrosin showed a developing of bronchiolitis obliterans in allograft 90 days after transplantation. Exp. 2. The development of fibrous airway obliteration was inhibited in group B rat which was administered proton pump inhibitor. However, This experimental model was not preferable for evaluating the efficacy of proton pump inhibitor in our study because the rate of occurrence in developing BO was not stable.
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