2001 Fiscal Year Final Research Report Summary
Single high-dose radiotherapy for experimental rat prolactinoma
| Project/Area Number |
11671353
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Gunma University |
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Principal Investigator |
TAKAHASHI Akio Gunma University, School of Medicine, Assist. prof., 医学部, 助手 (60261856)
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| Co-Investigator(Kenkyū-buntansha) |
NEGISHI Masatoshi Gunma University, School of Medicine, Assist. prof., 医学部, 助手 (80272235)
KOBAYASHI Satoshi Gunma University, School of Medicine, Assist. prof., 医学部, 助手 (00265779)
FUJIMAKI Hiroya Gunma University, School of Medicine, Assist. prof., 医学部, 助手 (80292613)
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| Project Period (FY) |
1999 – 2001
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| Keywords | experimental rat pituitary adenoma / single high-dose radiotherapy / prolactinoma |
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| Research Abstract |
Background : Single high-dose radiotherapy, i.e. gamma knife, is remarkable for the treatment for human prolactinoma. Objective and hypothesis : To clarify the hormonal and pathological effects of single high-dose radiotherapy for human prolactinoma, we used rat prolactinoma model. Method : A 5-week-old male Fischer 344 rat was subcutaneously implanted with 5 mg of diethylstilbestrol (DES) Seven weeks later, prolactinoma was induced and the tumor was treated with single high-dose radiotherapy with 10 mm collimator. Four weeks later, the rats were sacrificed, and weight of pituitary gland, serum prolactin(PRL) and Ki-67(MIB-5) labeling index were measured. Results : Of the control group (n=4), weight of pituitary gland was 7.2 mg, serum PRL was 35.8 ng/ml and MIB-5 labeling was 0.59 %. Of the rats with DES implant and without radiotherapy (n=5) weight of pituitary gland was 11.6 mg, serum PRL was 559 ng/ml and MIB-5 labeling was 0.88 %. Of the rats with DES implant and with 16 Gy radiotherapy (n=4), weight of pituitary gland was 11.1 mg, serum PRL was 121 ng/ml and MIB-5 labeling was 0.46 %. Of the rats with DES implant and with 32 Gy radiotherapy (n=5), weight of pituitary gland was 7.7 mg, serum PRL was 16.8 ng/ml and MIB-5 labeling was 0.38 %. Conclusions : Single high-dose radiotherapy is effective in the treatment for prolactinoma, dose-dependently. A dose of 32 Gy is recommended to practical use.
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