Anti-tumor Effect of Plasminogen Activator Inhibitor Derived from Shark Cartilage

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 11671455
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Keio University
• Principal Investigator: YABE Hiroo Keio University, School of Medicine, Lecturer, 医学部, 講師 (70119030)
• Co-Investigator(Kenkyū-buntansha): MORIOKA Hideo Keio University, School of Medicine, Assistant, 医学部, 助手 (10230096)
• Project Period (FY): 1999 – 2001
• Keywords: Cartilage / Shark / Angiogenesis / Plasminogen / Endothelial Cell / Plasminogen Activator Inhibitor / Plasminogen Activator / Arti-angiogenesis

Research Abstract

Anti-tumor substances were extracted from shark cartilage with 1M GuHCl and partially purified by ultrafiltration, gel-chromatography, affinity-chromatograry and SDS-PAGE. This extracted fraction was called as "Cartilage-derived plasminogen activator inhibitor: CD-PAI". The Physiological activity of the CD-PAI to endothelial cell and tumor cells were assayed by in vitro and in vivo study. In in vitro studies, the CD-PAI had inhibitory activities against endothelial cell proliferation, migration and tube formation. And also, the CD-PAI showed inhibitory activity against tumor cell invasion. On the other hand, tumor cell proliferation was not inhibited by the CD-PAI. In in vivo studies, the CD-PAI inhibited tumor growth implanted in mice. Theses results suggest that the tumor inhibitory mechanisms of the CD-PAI are anti-angiogenesis and anti-tumor invasion. We felt that further studies concerning with analysis of protein sequence and distribution of the CD-PAI in human normal tissue.