2000 Fiscal Year Final Research Report Summary
Analysis and Quantification on Immunosuppressive Effects of Inhalation Anesthetics
| Project/Area Number |
11671475
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Tohoku University |
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Principal Investigator |
KATO Masato Tohoku University, Graduate School of Med., Associate Professor (50169520)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUOKA Hiroshi Tohoku University, Tohoku University Hospital, Research Associate (40302111)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Apoptosis / Programmed Cell Death / Inhalational Anesthetics |
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| Research Abstract |
The authors hypothesized that perioperative lymphocytopenia is partially caused by apoptosis of lymphocytes induced by inhalation anesthetics. Therefore, they evaluated whether sevoflurane and isoflurane induce apoptosis of normal peripheral lymphocytes.
Methods: Normal peripheral blood mononuclear cells were exposed to sevoflurane and isoflurane, and the percentages of apoptotic lymphocytes was measured by Annexin V-fluorescein isothiocyanate-7-amino actinomycin D flow cytometry after 24h of exposure(0.5,1.0,and 1.5mm) and after 6, 12, and 24h of exposure(1.5mm). The percentages of lymphocytes with caspase 3-like activity were also measured after 24h of exposure(1.5mm).
Results : The percentages of apoptotic lymhocytes were increased in a dose-dependent manner(controls: 5.1±1.4%; sevo-flurane: 7.3±1.3% [0.5mm], 9.1±1.5% [1.0mm], 12.6±2.1% [1.5mm]; isoflurane: 7.5±1.6% [0.5mm], 10.5±1.5% [1.0mm], 16.3±2.7% [1.5mm]) after 24h of exposure and in a time-dependent manner ( controls: 1.2±0.4% [6h], 3.4±0.7% [12h], 5.6±1.2% [24h]; sevoflurane: 1.8±0.4% [6 h], 6.4±1.2% [12h], 11.3±2.2% [24±h]; isoflurane: 2.6±0.5% [6h],8.8±1.5% [12h],16.0±1.9% [24h]) at the concentration of 1.5mm. The percentages of lymphocytes with caspase 3-like activity were increased (controls: 10.0±1.1%; sevoflurane: 13.8±1.2%; isoflurane: 17.0±1.3%).
Conclusions : Both sevoflurane and isoflurane induced apoptosis in peripheral lymphocytes in
dose-dependent and time-dependent manners in vitro.
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[Presentation] Propofol Dilates Rat Intracerebral Arterioles by Inhibition of Voltage-Dependent Ca 2+ Channels2000
Author(s)
Yasuhiko Furuyama, MD, PhD, Yoshiaki Kondo, MD, PhD, Shoko Kawaguchi, MD, Mamoru Murakami, MD, Masato Kato, MD
Place of Presentation
San Francisco
Year and Date
2000-10-16
Description
「研究成果報告書概要(欧文)」より
