2001 Fiscal Year Final Research Report Summary
Changes in β-adrenergic receptor-mediated vascular response in the septic rat thoracic aorta
| Project/Area Number |
11671515
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
TSUCHIDA Hideaki Kanazawa Medical University, Department of Anesthesiology, Professor, 医学部, 教授 (20155394)
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| Project Period (FY) |
1999 – 2001
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| Keywords | rat / aorta / sepsis / endothelium-dependent vasorelaxation / β-adrenergic receptor / muscarinic receptor / Ca concentration / halothane |
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| Research Abstract |
The mechanism (s) underlying the inhibitory effect of anesthetics or sepsis on endothelium-dependent vasorelaxation is still controversial. This study intended to clarify how anesthetics or sepsis interfered with endothelium-mediated vasorelaxation.
The volatile anesthetic halothane inhibited carbachol-induced increase in cytosolic Ca^<2+> concentration and resultant decrease in muscle tension in aconcentration-dependent manner. In addition,halothane concentration dependently inhibited nitroglycerin-induced vasorelaxation partly in a Ca^<2+>-dependent fashion. These findings strongly indicated that halothane interfered with endothelium-mediated vasorelaxation both at the NO synthetic process and at the NO-mediated vasorelaxing process. Dobutamine, a β1 adrenergic agonist, and salbutamol, a β2 adrenergic agonist, induced vasorelaxation in endothelium-intact vascular strip. In contrast, dobutamine's effect was almost abolished in endothelium-denuded strip, suggesting that dobutamine's effect was mediated by endothelium. Not only α-adrenergic receptor-mediated vasoconstriction, but β-adrenergic receptor-mediated vasorelaxation was inhibited in the septic rat aorta. Therefore, sepsis induces multifactorial inhibition on vascular relaxation.
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Research Products
(15 results)
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[Publications] Kanaya,N., Kimura,H., Nakayama,M., Tsuchida,H., Ohshika,H., Namiki,A.: "The direct effect of halothane on myocardial contraction in rat myocytes with poorly developed gap junctional intercellular communication"Acta Anaesthesiol Scand. 43. 91-6 (1999)
Description
「研究成果報告書概要(欧文)」より
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