• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2000 Fiscal Year Final Research Report Summary

Basic study for the prevention of recurrence by inhibition of cell adhesion and migration in superficial bladder cancer.

Research Project

Project/Area Number 11671533
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionHokkaido University

Principal Investigator

HARABAYASHI Toru  Hokkaido Univ., Medical, Hospital, Inst., 医学部・附属病院, 助手 (70301900)

Co-Investigator(Kenkyū-buntansha) SHINOHARA Nobuo  Hokaido Univ., Medical, Hospital, Lect., 医学部・附属病院, 講師 (90250422)
Project Period (FY) 1999 – 2000
Keywordsbladder cancer / recurrence / intravesical dissemination / cell-extracellular matrix adhesion / integrin / orthotopic transplantation model / intracellular signaling pathway
Research Abstract

Superficial bladder cancer is characterized by its multiplicity and recurrence, which is supposed to be caused by intraluminal dissemination. In this step, adhesion and migration of cancer cells on extra cellular matrices mediating through membranous protein and intracellular signaling pathways are of great importance.
1) To examine in vivo intraluminal dissemination, we developed a murine orthotopic implantation model of bladder cancer. After the pretreatment by EDTA, cancer cell suspensions were injected to the murine bladder through the urethral catheter and kept in the bladder by 3-hour urethral ligation. The ability of intraluminal dissemination was examined in the bladder cancer cell lines using this method. The 6 cell lines were classified into 3 groups ; highly growing cell lines (UMUC-2 : 100%, KU7 : 100% and UMUC-6-dox : 100%), and a low growing cell line (EJ : 18%), and no growing cell lines (KU-1 : 0%, DAB-1 : 0%). 2) Expressions of cell adhesion molecules, adhesion mediating signaling molecules were examined by Western blot analysis. Highly growing cell lines showed that reduction of integrin beta-4, cadherin and beta-catenin, although the expressions of FAK, paxillin, alpha-catenin, Csk and Crk varied between 3 groups. From these results, the expression of integrin beta-4 and cadherin played an important role in intraluminal dissemination. 3) The expression and distribution of integrin beta-4 was examined using surgically resected specimen from bladder cancer patients. The expression and distribution of integrin beta-4 was strongly correlated with T-stage and metastasis. These results suggested that control of cell adhesion protein and mediating signals could inhibit the recurrence of the bladder cancer.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Toru Harabayashi et al.: "Reduction of integrin β4 and enhanced migration of lamimn in association with intraepithelial spreading of urinary bladder carcinomas."J.Urol.. 161. 1364-1371 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takafumi Watanabe et al.: "An improved intravesical model using human bladder cancer cell lines to optimize gene and other therapies."Cancer Gene Therapy. 7. 1575-1580 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toru Harabayashi, Yae Kanai, Tesshi Yamada, Michiie Sakamoto, Atsushi Ochiai, Tadao Kakizoe, Tomohiko Koyanagi, Setsuo Hirohashi: "Reduction of integrin beta-4 and enhanced migration of laminin in association with intraepithelial spreading of urinary bladder carcinomas."J.Urol.. 161. 1364-1371 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takafumi Watanabe, Nobuo Shinohara, Ataru Sazawa, Toru Harabayashi, Yoshifumi Ogiso, Tomohiko Koyanagi, Mitsuyoshi Takiguchi, Akira Hashimoto, Noboru Kuzumaki, Motoyuki Yamashita, Motoyoshi Tanaka, H.Barton Grossman, William F.Benedict: "An improved intravesical model using human bladder cancer cell lines to optimize gene and other therapies."Cancer Gene Therapy. 7. 1575-1580 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2002-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi