2000 Fiscal Year Final Research Report Summary
Identification of the target molecules for antiangiogenic therapy
| Project/Area Number |
11671561
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Nagasaki University |
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Principal Investigator |
KANDA Shigeru Graduate School of Medicine, Nagasaki University Associate Professor, 大学院・医学研究科, 助教授 (20244048)
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| Co-Investigator(Kenkyū-buntansha) |
OTSURU Akira School of Medicine, Nagasaki University Assistant Professor, 医学部, 助手 (00233198)
NOMATA Koichiro University Hospital, Nagasaki University, Lecturer, 医学部・附属病院, 講師 (80189430)
KANETAKE Hiroshi School of Medicine, Nagasaki University Professor, 医学部, 教授 (50100839)
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| Project Period (FY) |
1999 – 2000
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| Keywords | endothelial cells / angiogenesis / fibroblast growth factor receptor / anti-angiogenic therapy / c-Src / c-Fes / c-Fyn / tube formation |
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| Research Abstract |
Head investigator has established a murine brain capillary endothelial cells (IBE cells) in 1996 and started the examination of FGF-2-mediated signal transduction pathways leading to angiogenesis. The purpose of the study is to identify specific signaling molecules involved in angiogenesis to make it possible to establish a new drug specifically and potently inhibit angiogenesis in vivo, such as tumor growth. IBE cells exhibit a panel of angiogenic cellular responses, such as urokinase production, proliferation, migration and tube formation. However, tube formation is rather specific for endothelial cells when compared with malignant tumor cells. By using this culture model, we found several important molecules as follows ;
a. Blocking tube formation without any effect on proliferation by 4N1K peptide was enough to inhibit in vivo angiogenesis (mouse cornea micropocket assay). This result suggests the possibility that targeting tube formation is a good antiangiogenic strategy.
b. c-Fes tyrosine kinase was involved in FGF-2-mediated chemotaxis and overexpression of wild type c-Fes induced FGF-2-independent tube formation.
c. c-Src kinase was involved in chemotaxis toward FGF-2 by activating mitogen-activated protein kinase within focal adhesions.
d. c-Fyn was transiently activated in IBE cells when cultured only on collagen gels (tube forming condition without proliferation or migration) and three stable cell lines expressing kinaseinactive c-Fyn exhibited attenuation of FGF-2-nediated tube formation (submitted). In the same study, it was found that c-Fes was activated by the adhesion of collagen gels. More recently, another growth factor was found to induce migration and tube formation. For migration, c-Fes was used and c-Fyn played a pivotal role in tube formation (submitted). Taken together, downstream molecules of c-Fes and c-Fyn would be potential targets of antiangiogenic therapy.
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