2000 Fiscal Year Final Research Report Summary
Role of metallothionein on carcinogenesis and tumor progression in bladder cancer.
Project/Area Number |
11671586
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Urology
|
Research Institution | Nippon Medical School |
Principal Investigator |
KONDO Yukihiro Nippon Medical School, Dept. of Urology, Associate Professor, 医学部, 助教授 (80215467)
|
Co-Investigator(Kenkyū-buntansha) |
AKIMOTO Masao Nippon Medical School, Dept. of Urology, Professor, 医学部, 教授 (50089752)
|
Project Period (FY) |
1999 – 2000
|
Keywords | bladder cancer / metallothionein / carcinogenesis / tumor progression |
Research Abstract |
(1) Immortalized metallothionein null cells Immortalized metallothionein(MT) null cells were established by transfection of simian virus 40 into MT null fibroblast. Additionally, immortalized control fibroblast were established. Immortalized MT null cells showed increased sensitivity cadmium not to zinc, copper, mercury or nickel. These results were written in Life Science, 64,1999,145-150. (2) Metallothionein prevents the carcinogenicity. We examined the carcinogenicity of N-butyl-N-(4-hydroxybutyl) nitrosamine(BBN) in transgenic mice deficient in the MT I and II genes and control (129/Sv) mice. BBN induced bladder tumors in 75% of MT null mice and in 43% of 129/Sv mice. The average number of bladder tumors per mouse was significantly higher in MT null mice than in 129/Sv mice. Zinc treatment suppressed the carcinogenicity of BBN in 129/Sv mice but not in MT null mice. These results were written in Carcinogenesis, 20, 1999, 1625-1627. (3) Histopathologic examination Histopathologic examination revealed that all tumor samples showed morphological changes characteristic of transitional cell carcinoma in both MT null and 129/Sv mice. The major bladder tumors in 129/Sv mice displayed the features of high grade, whereas none of the tumors in MT null mice were designated as grade III.Thus, the malignant potential of bladder tumors in 129/Sv mice was greater than that in MT null mice. (4) MT null bladder tumor cell line We established MT null and 129/Sv bladder tumor cell line. MT null bladder tumor cells were unable to induced MT by administered cadmium. These cells were sensitive to cadmium and cisplatin against 129/Sv bladder tumor cells.
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[Publications] Kondo, Y., Yanagiya, T., Himeno, S., Yamabe, Y., Schwartz, D., Akimoto, M., Lazo, J.S.and Imura, N.: "Simian virus 40-transformed metallothionein null cells showed increased sensitivity to cadmium but not to zinc, copper, mercury or nickel."Life Science. 64. 145-150 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Sakurai, A., Hara, S., Okano, N., Kondo, Y., Inoue, J.and Imura, N.: "Regulatory role of metallothionein in NF-kB activation."FEBS Letter. 455. 55-58 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Nemoto, K., Kondo, Y., Himeno, S., Suzuki, Y., Hara, S., Akimoto, M and Imura, N.: "Modulation of telomerase activity by zinc in human prostatic and renal cancer cells."Biochemical Pharmacology. 59. 401-405 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Kondo, Y., Himeno, S., Endo, W., Mita, M., Suzuki, Y., Nemoto, K., Akimoto, M., Lazo, J.S.and Imura, N.: "Metallothionein modulates the carcinogenicity of N-butyl-N-(4-hydroxybutyl) nitrosamine in mice."Carcinogenesis. 20. 1625-1628 (1999)
Description
「研究成果報告書概要(欧文)」より
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