1999 Fiscal Year Annual Research Report

卵巣における新しいプロラクチン放出ペプチド作用機構の機能的組織学的研究

Research Project

Project/Area Number	11671596
Research Institution	University of Tsukuba
Principal Investigator	臼杵哲筑波大学, 臨床医学系, 助教授 (50092151)
Co-Investigator(Kenkyū-buntansha)	塩田正樹熊本大学, 理学部, 教授 (80134526)
Keywords	PrRP / 卵巣機能 / ゴナドトロピン / PACAP / ERAANPS / Endothelin / Angiotensin / DNA Polymerase
Research Abstract	新しく発見されたProlactin(PRL)-releasing peptide(PrRP)の卵巣機能に及ぼす作用機序の解明は不明で,PrRPの卵巣機能に及ぼす直接的・間接的作用を解明する目的で本研究を進めた。今回はIn vivoおよびIn vitroでの臓器レベルでの研究を主に行った。まず,PrRPの卵巣に於ける存在を明らかにした。さらに,PrRPの卵胞の発育と成熟,排卵および黄体の形成と退行に果たす役割を,In vivoないし各時期に採取したラット卵巣細胞のIn vitro培養液にPrRPを投与することによってホルモン産生能,レニン-アンジオテンシン-atrial natriuretic peptide syatem(ERAANPS),Adenylate cyclase系,Cyclic nucleotides(Cyclic AMP,Cyclic GMP),Calcium Channel,Insulin-like growth factor(IGF/ソマトメジンC),DNA合成酵素,DNA合成系および遺伝子レベルから検討した。Programmed cell death(Apoptosis)に及ぼす影響についても明らかにした。同時に,PrRPのLH,FSH,プロラクチン,PACAP,GHおよびVIP作用に及ぼす影響についても明らかにした。 PrRPは卵巣機能に重要な影響を及ぼしているおり,われわれが先に提唱したERAANPSやGHと深く関わり,Steroidogenesis,DNA Polymerase a活性などの卵巣機能調節に深く関わっていることが明らかにされてきている。概略的には計画的に進んでいる。

Research Products

(8 results)

All Other

All Publications (8 results)

[Publications] Usuki S, Kondoh K, et al.: "Pituitary Adenylate Cyclase-activating Polypeptide Stimulates the Mitogen-activating Kinases by Granulosa Cells. Recent Advances in Endocrinology"Hormone-Receptor Interaction and Signaling. (in press.).
[Publications] Usuki S, Kondoh K, et al.: "Plasma endothelinand LH-RH,LH,FSH, Prolactin,…of the Normal Cycling Women"J. Cardiovasc. Pharmacol. (in press.).
[Publications] Kotani E, Usuki S, et al: "Pituitary Adenylate Cyclase-activating Polypeptide (PACAP) in the Rat Ovary"Control Mechanisms of Stress and Emotion in Occupational and Environmental Health: Neuroendocrine Based Studies, Proceedings of 18th UOEH International Symposium. (in press.).
[Publications] Kotani E, Sugimoto M. ,et al.: "Biological Roles of Angiotensin II via Its Type 2 Receptor during Rat Follicle Atresia."Am. J. Physiol.. 276. E25-E33 (1999)
[Publications] Fu Y., Asano K. ,et al.: "Serum Testosterone Responses to Continuous and Intermittent Exercise Training in Male Rats."Int. J. Sport. Med.. 20. 12-16 (1999)
[Publications] Usuki., S, Kondoh K. ,et al.: "GH and IGF-1 Effect on Cultured Granulosa Cells fron fron Rat Ovary."Recent Advances in Molecular and Comparative Endocrinology,. 528-532 (1999)
[Publications] Kotani E.,Usuki S,et al.: "Frontiers in Endocrinology; Overian Function Research:Present and Future,21"Pituitary Adenylate Cyclase-activating Polypeptide in the Rat Ovary.. 99-108 (1999)
[Publications] Kondoh K,Usuki S,et al.: "Recent Advances in Molecular and Comparative Endocrinology"FSH Effect on Endothelin Receptors in Granulosa Cells from Rat Ovary.. 92-98 (1999)

1999 Fiscal Year Annual Research Report

卵巣における新しいプロラクチン放出ペプチド作用機構の機能的組織学的研究

Principal Investigator

臼杵 哲 筑波大学, 臨床医学系, 助教授 (50092151)

Research Products

[Publications] Usuki S, Kondoh K, et al.: "Pituitary Adenylate Cyclase-activating Polypeptide Stimulates the Mitogen-activating Kinases by Granulosa Cells. Recent Advances in Endocrinology"Hormone-Receptor Interaction and Signaling. (in press.).

[Publications] Usuki S, Kondoh K, et al.: "Plasma endothelinand LH-RH,LH,FSH, Prolactin,…of the Normal Cycling Women"J. Cardiovasc. Pharmacol. (in press.).

[Publications] Kotani E, Usuki S, et al: "Pituitary Adenylate Cyclase-activating Polypeptide (PACAP) in the Rat Ovary"Control Mechanisms of Stress and Emotion in Occupational and Environmental Health: Neuroendocrine Based Studies, Proceedings of 18th UOEH International Symposium. (in press.).

[Publications] Kotani E, Sugimoto M. ,et al.: "Biological Roles of Angiotensin II via Its Type 2 Receptor during Rat Follicle Atresia."Am. J. Physiol.. 276. E25-E33 (1999)

[Publications] Fu Y., Asano K. ,et al.: "Serum Testosterone Responses to Continuous and Intermittent Exercise Training in Male Rats."Int. J. Sport. Med.. 20. 12-16 (1999)

[Publications] Usuki., S, Kondoh K. ,et al.: "GH and IGF-1 Effect on Cultured Granulosa Cells fron fron Rat Ovary."Recent Advances in Molecular and Comparative Endocrinology,. 528-532 (1999)

[Publications] Kotani E.,Usuki S,et al.: "Frontiers in Endocrinology; Overian Function Research:Present and Future,21"Pituitary Adenylate Cyclase-activating Polypeptide in the Rat Ovary.. 99-108 (1999)

[Publications] Kondoh K,Usuki S,et al.: "Recent Advances in Molecular and Comparative Endocrinology"FSH Effect on Endothelin Receptors in Granulosa Cells from Rat Ovary.. 92-98 (1999)

臼杵哲筑波大学, 臨床医学系, 助教授 (50092151)