2001 Fiscal Year Final Research Report Summary
The expression and function of the small GTPase Rho in the oocyte during fertilization and cell division
| Project/Area Number |
11671618
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University |
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Principal Investigator |
SAKATA Masahiro OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE, lecturer, 医学系研究科, 講師 (10260639)
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| Co-Investigator(Kenkyū-buntansha) |
MORISHIGE Ken-ichiro Osaka University Graduate School of Medicine, Associate professor, 医学系研究科, 助手 (90283788)
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| Project Period (FY) |
1999 – 2001
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| Keywords | G-protein / Rho / blastcyst / morula / 受精 / 細胞分裂 |
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| Research Abstract |
The small GTPase Rho is involved in various cellular functions including migration, proliferation and smooth muscle contraction. To identify the Rho protein and mRNA in the oocyte, we performed immunoblot analysis and RT-PCR method. We detected the Rho protein and mRNA in the oocyte. However, we could not the change in the level of the protein and mRNA in the oocyte during fertilization. The specific Rho-kinase inhibitor had the inhibitory effect on the progress from morula to blastcyst.
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