• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2000 Fiscal Year Final Research Report Summary

Role of superoxide dismutase in the prolongation of the life span of the corpus luteum

Research Project

Project/Area Number 11671623
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionYamaguchi University

Principal Investigator

SUGINO Norihiro  Yamaguchi University School of Medicine, Research Associate, 医学部, 助手 (10263782)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Yasuhiko  Yamaguchi University Hospital, Associate Professor, 医学部・附属病院, 助教授 (30227946)
Project Period (FY) 1999 – 2000
Keywordscorpus luteum / SOD / superoxide radical / apoptosis / life span / progesterone / testosterone / placental luteotropin
Research Abstract

1. The first study was undertaken to investigate whether suppression of intracellular Cu, Zn-SOD activity influences luteal function. To suppress SOD activity, dispersed rat luteal cells were incubated with Cu, Zn-SOD antisense oligonucleotides. The 48-h treatment with antisense oligonucleotides (10 μM) inhibited both SOD activity by 50% and progesterone concentrations in the medium. This inhibitory effect was completely blocked by the simultaneous addition of an antioxidant. Treatment with antisense oligonucleotides caused no significant change in the percentage of apoptotic cells. It is concluded that the decrease in intracellular SOD activity inhibits progesterone production by rat luteal cells, which may be mediated by superoxide radicals, suggesting that intracellular SOD plays important roles in the regulation of luteal function.
2. (1) The second study was undertaken to investigate whether SOD is related to pregnancy-induced maintenance of luteal function. Placentae obtained from … More rats on day 12 of pregnancy were incubated for 24h, and the supernatant was used as placental luteotropins. Pseudopregnant rats were given the placental incubation medium from day 9 to day 12 of pseudopregnancy. Serum progesterone concentrations and luteal SOD mRNA and activity were increased by this treatment on day 12. Testosterone in the placental incubation medium acted on the corpus luteum without conversion to estrogen. (2) The third study was undertaken regarding the involvement of SOD in the human luteal function. SOD expression in the corpus luteum of early pregnancy was significantly higher than that in the mid-luteal phase corpus luteum. The incubation of the mid-luteal phase corpus luteum with hCG increased both luteal SOD expression and progesterone production. Collectively, it is concluded that placental luteotropins increased SOD expression in the corpus luteum and stimulated progesterone production, suggesting that SOD is involved in the maintenance of the luteal function by placental luteotropins. Less

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Sugino N: "Suppression of intracellular superoxide dismutase activity by antisense oligonucleotides causes inhibition of progesterine production by rat luteal cells."Biology of Reproduction. 61. 1133-1138 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takiguchi S.: "Rescue of the corpus luteum and an increase in luteal superoxide dismutase expression induced by placental luteotropins in the rat : action of testosterone without conversion to estrogen."Biology of Reproduction. 62. 398-403 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugino N: "Superoxide dismutase expression in the human corpus luteum during the menstrual cycle and in early pregnanal."Molecular Human Reproduction. 6. 19-25 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugino N.: "Expression of vascular endothelial growth factor and its receptors in the human corpus luteum during the menstrual cycle and in early pregnancy."Journal of Clinical Endocrinology and Metabolism. 85. 3919-3924 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugino N.: "Expression of Bcl-2 and Bax in the human corpus luteum during the menstrual cycle and in early pregnancy regulation by human chorionic gonadotropin."Journal of Clinical Endocrinology and Metabolism. 85. 4379-4386 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugino N.: "Induction of superoxide dismutase by decidualization in human endometrial stromal cells."Molecular Human Reproduction. 6. 178-184 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugino N: "Decreased superoxide dismutase. expression and increased concerntrations of lipid peroxide and prostaglandin F12α in the decidum of failed pregnanoy."Molecular Human Reproduction. 6. 642-647 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kashida S.: "Regulation and role of vascular endothelial growth factor in the corpus luteum during mid-pregnancy in rats."Biology of Reproduction. 64. 317-323 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tashima L.: "Prenatal influence of ischemin-hypoxia induced intrauterine growth retardation of brain development and behavioral activity in rats."Biology of the Neonate. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugino N.et al.: "Suppression of intracellular superoxide dismutase activity by antisense oligonucleotides causes inhibition of progesterone production by rat luteal cells."Biology of Reproduction. 61. 1133-1138 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takiguchi S.et al.: "Rescue of the corpus luteum and an increase in luteal superoxide dismutase expression induced by placental luteotropins in the rat : action of testosterone without conversion to estrogen."Biology of Reproduction. 62. 398-403 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugino N.et al.: "Superoxide dismutase expression in the human corpus luteum during the menstrual cycle and in early pregnancy."Molecular Human Reproduction. 6. 19-25 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugino N.et al.: "Expression of vascular endothelial growth factor and its receptors in the human corpus luteum during the menstrual cycle and in early pregnancy."Journal of Clinical Endocrinology and Metablism. 85. 3919-3924 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugino N.et al.: "Expression of Bcl-2 and Bax in the human corpus luteum during the menstrual cycle and in early pregnancy : regulation by human chorionic gonadotropin."Journal of Clinical Endocrinology and Metabolism. 85. 4379-4386 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugino N.et al.: "Induction of superoxide dismutase by decidualization in human endometrial stromal cells."Molecular Human Reproduction. 6. 178-184 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugino N.et at.: "Decreased superoxide dismutase expression and increased concentrations of lipid peroxide and prostaglandin F2a in the decidua of failed pregnancy."Molecular Human Reproduction. 6. 642-647 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kashida S.et al.: "Regulation and role of vascular endothelial growth factor in the corpus luteum during mid-pregnancy in rats."Biology of Reproducation. 64. 317-323 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tashima L.et al.: "Prenatal influence of ischemia-hypoxia induced intrauterine growth retardation of brain development and behavioral activity in rats."Biology of the Neonate. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2002-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi