2002 Fiscal Year Final Research Report Summary
The mechanisms of follicular development, ovulation and atresia by intraovarian regulating factor
| Project/Area Number |
11671647
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kyorin University |
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Principal Investigator |
ANDO Motomu Kyorin University School of Medicine Dept. OB/GYN, Assoc. Prof., 医学部, 助手 (10301483)
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| Project Period (FY) |
1999 – 2002
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| Keywords | Ovary / Ovulation / Interleukin-1 / Cyclooxygenase / interleukin-6 / VEGF / ICE |
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| Research Abstract |
Interleukin (IL)-1, a putative intermediary in the ovulatory process, is capable of upregulating prostaglandin biosynthesis by cultured whole ovarian dispersates from immature rats. It was the purpose to examine the posibility that the prostaglandin-promoting property of IL-1 is also due to the actination of prostaglandin endoperoxide synthase (PGS), the rate-limiting step in prostaglan-din biosynthesis, IL-1βconverting enzyme (ICE), a putative apoptotic marker, vascular endothelial growth factor (VEGF), an endothelial cell mitogen and permiability factor and IL-6, traditionally an IL-1-induced immune regulator.
Whole ovarian dipersate from immature rats were cultured under serum free condition for up to 48h in the absence or presence of indicates agents. At the conclusion of the culture period, total RNA was extracted and subjected to a solution hybridization/ribonuclease protection assay. Ribosomal protein L19 was used as an internal control.
A periovulatory increase in ovarian ICE gene expression and in vivo increase in PGS-2, VEGF and IL-6 before ovulation were documented. The IL-1β effect to be receptor mediated and dose and time dependent, contingent upon de novo protein biosynthesis were revealed by each factor. These findings suggest that IL-1 action in the ovary may require the intermediary of VEGF and IL-6. Ovarian ICE was also suggested a role in the context of apoptosis/atresia in the ovulatory process.
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