2001 Fiscal Year Final Research Report Summary
Localization of peptides that regulate inner ear fluid in the rat
| Project/Area Number |
11671676
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
KITANO Hiroya Shiga University of Medical Science, Department of Otolaryngology, Assistant Professor, 医学部, 講師 (20153108)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Hiroshi Shiga University of Medical Science, Department of Molecular Neurobiology Research center, Professor., 分子神経科学研究センター, 教授 (40079736)
SUZUKI Mikio Shiga University of Medical Science, Department of Otolaryngology, Assistant Professor., 医学部, 講師 (00226557)
YAZAWA Yoshiro Shiga University of Medical Science, Department of Otolaryngology, Associate Professor., 医学部, 助教授 (60127040)
TOOYAMA Ikuo Shiga University of Medical Science, Department of Molecular Neurobiology Research center, Professor., 分子神経科学研究センター, 教授 (20207533)
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| Project Period (FY) |
1999 – 2001
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| Keywords | inner ear / Meniere's disease / Water regulation / endolymphatic hydrops / vasopressin / CNP / molecular biology / vertigo |
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| Research Abstract |
The anti-diuretic hormone, vasopressin, has been shown to be important in regulating inner ear fluid. The diuretic hormone, CNP, and its receptor, ANP-B receptor, may also function in the regulation of inner ear fluid. To determine whether vasopressin directly affects the fluid level, we infused this hormone to rat and assay of V2-AVP receptor mRNA by semiquantitative RT-PCR demonstrated a significantly lower level of this transcript in vasopressin-infused animals than in saline-infused animals. The levels of CNP and ANP-B receptors mRNA, however, were the same in both groups of rats. Localization of vasopressin V2 receptor was identified by insitu hybridization. And we chronically infused this hormone to rats and examined them for morphological signs of endolymphatic hydrops. Enlargement of the scala media area was observed in the vasopressin-infused rats. The results suggest that high plasma levels of vasopressin may be a principal causal factor of endolymphatic hydrops in Meniere's disease, perhaps by down-regulating the number of vasopressin receptors.
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