2000 Fiscal Year Final Research Report Summary
Role of osteopontin in the calcification of chronic otitis media
| Project/Area Number |
11671714
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
SAKAGAMI Masafumi Medical School, Professor, 医学部, 教授 (10170573)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJIMURA Tohru Medical School, Associate Professor, 医学部, 助教授 (20227408)
FUKAZAWA Keijiro Medical School, Associate Professor, 医学部, 助教授 (50278842)
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| Project Period (FY) |
1999 – 2000
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| Keywords | chronic otitis media / calcification / tympanosclerosis / osteopontin / macrophage / tymapanic membrane / tympanic mucosa / in situ hybridization |
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| Research Abstract |
Tympanosclerosis is a condition leading to a calcification process in the middle ear, and often develops after chronic inflammation of the middle ear. Since osteopontin (OPN) has been shown to participate in the pathological calcification, we here investigated whether OPN is involved in the process of calcification in tympanosclerosis. The tympanic membrane and middle ear mucosa, obtained from patients of tympanosclerosis and chronic otitis media, were histologically classified depending on the calcification degree. In hyalinized tissues with macroscopic calcification and fibrous tissues with microscopic calcification, OPN was also found in the calcifying foci, and many OPN mRNA-expressing cells, determined by in situ hybridization, located around their foci. Moreover, immunohistochemical double staining of OPN and CD68 showed that the OPN-expressing cells were CD68-positive indicating these cells were macrophages. In inflammatory tissues without calcification, immunohistochemistry of CD68 and in situ hybridization of OPN mRNA revealed that most OPN mRNA-expressing cells were CD68-positive. The expression of OPN mRNA in inflammatory tissues was also shown by reverse transcriptase polymerase chain reaction. These results suggested that OPN secreted by exudate macrophages might be an important regulator in the calcification of tympanosclerosis.
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