2000 Fiscal Year Final Research Report Summary
Role for corneal fibroblasts in the pathogenesis of allergic conjunctival diseases
| Project/Area Number |
11671741
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
KUMAGAI Naoki Yamaguchi University Hospital Assistant Professor, 医学部・附属病院, 講師 (20234510)
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| Project Period (FY) |
1999 – 2000
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| Keywords | Corneal fibroblasts / eotaxin / thymus and activation-regulated chemokines / TNG-α / IL-4 / IL-13 |
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| Research Abstract |
1. Synergistic induction of eotaxin expression by corneal fibroblast by TNF-α and IL-4 or IL-13.
The effect of TNF-α, IL-4 or IL-13 on the expression of chemokine eotaxin by cultured human corneal fibroblasts were examined by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). Without the stimulation by cytokines, the expression of eotaxin protein by these cells was below detectable level. These cells expressed eotaxin protein by the stimulation of TNF-α, IL-4 or IL-13 in a dose-dependent manner. The expression of eotaxin protein and mRNA was synergistically increased by the stimulation of TNF-α and IL-4 or IL-13.
2. Synergistic induction of thymus and activation-regulated chemokine (TARC) by TNF-α and IL-4
The effect of TNF-α and IL-4 on the expression of chemokine TARC by cultured human corneal fibroblasts were examined by ELISA and quantitative RT-PCR.
Corneal fibroblast did not express detectable TARC protein or mRNA spontaneously or by the stimulation of TNF-α or IL-4. When these cells were stimulated in the presence of both TNF-α and IL-4, these cells expressed TARC protein and mRNA.
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