• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2001 Fiscal Year Final Research Report Summary

Establishment of experimental local gene therapy model for uveitis

Research Project

Project/Area Number 11671743
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

KAWANO Yoh-ichi  Kyushu university, Department of Ophthalmology, Lecturer, 医学部・附属病院, 講師 (90211185)

Project Period (FY) 1999 – 2001
KeywordsUveitis / gene therapy / adeno virus / VEGF / CD8 / CCR2
Research Abstract

Uveitis has become increasingly recognized as a major cause of blindness, especially in the working age population. The main cause of the irreversible damage of the eye depends on the relapse of the inflammation which is hard to suppress with the immunosuppressive agents in some entities of uveitis. The aim of the study of this period was to establish experimental model of the gene therapy for uveitis, targeted for the functional molecules that is involved for the cellular immune reaction.
We initially tested the efficacy of the LacZ gene transfer by adenovirus vecter in rat. Injection of the vector into anterior chamber and the vitreous cavity caused gene expression in angle and retinal ganglion cells but not in ciliary body and choroid. Injection into the eye with experimental uveitis decreased efficacy of the transfer. Subretinal injection of the vector caused gene expression in RPE and infiltrated leucocytes. These results showed the necessity of the novel methods for the gene transfer into uveal tissue.
To find out the effect of the genetical modulation of the functional molecules involved in the inflammation on uveitis, we tested the susceptibility for EAU induction in the CD8 KO mice and CCR2 KO mice. EAU were accerelated in CD8 KO mice, and the type of infiltrating innflammatory cells were changed in CCR2 KO mice. These results show the possibilty of the immunomodulation by the gene therapy targeted for these molecules.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Satomi Shiose: "Gene transfer of a soluble receptor of VEGF inhibits the growth of experimental eyelid malignant melanoma"Investigative ophthalmology & Visual Science. 41. 2395-2403 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 川野庸一: "ペーチェット病眼病変に対するステロイド薬長期間継続併用投与"眼科. 42. 421-428 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 川野庸一: "トキソプラズマ"眼科. 42. 1562-1566 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 坂本泰二: "悪性腫瘍と眼科的疾患の遺伝子治療"細胞学. 19. 468-475 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 川野庸一: "猫ひっかき病の眼病変"眼科. (印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 川野庸一: "水晶体起因性眼内炎"眼科 NEW MOOK. (印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 川野庸一(分担執筆): "ペーチェット病 眼の細胞生物学(沖坂重邦他 編)"中山書店. 5 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satomi Shiose, Taiji Sakamoto, Hiroshi Yoshikawa, Yasuaki Hata, Yoh-Ichi Kawano, Taturo Ishibashi, Hajime Inomata, Koichi Takayama and Hikaru Ueno: "Gene transfer of a soluble receptor of VEGF inhibits the growth of experimental eyelid malignant melanoma"Investigative ophthalmology & Visual Science. 41 (in press). 2395-2403 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoh-Ichi Kawano, Yuko Nishioka, Satomi Ashizuka, Takao Oniki, Sei-ichi Ishimoto, Hajime Inomata: "Long term low dose corticosteroid treatment for ocular Behchet's disease"Ganka. 42 (in press). 421-428 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoh-Ichi Kawano, Hajime Inomata: "Behchet's disease"Ocular cell biology. Nakayama shoten, Tokyo. 188-191 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoh-Ichi Kawano: "Toxoplasmosis"Ganka. 42. 1562-1566 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2003-09-17  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi