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2000 Fiscal Year Final Research Report Summary

Analysis of neural mechanisms of transformation from sucking to mastication in in vitro brain preparations.

Research Project

Project/Area Number 11671837
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KATAKURA Nobuo  Tokyo Medical and Dental University Department of Maxillofacial Biology, Section of Cognitive Neurobiology, Research Associate, 大学院・医歯学総合研究科, 助手 (20185804)

Project Period (FY) 1999 – 2000
Keywordssucking / mastication / mice / isolated brain preparation / NMDA / rhythm generation / hypoglossal nerve / trigeminal nerve
Research Abstract

Feeding behavior of mammals changes from sucking to mastication, coinciding with the teeth eruption. In adult mammals, it is thought that the neurons involved in the rhythm generation of mastication locate in the lower brainstem and distributed along rostro-caudal axis. The purpose of this study was to investigate the neural organization of the rhythm generation of sucking in in vitro preprarations. Rhythmical sucking-like activity (RSA) is known to be induced in the hypoglossal nerve (XIIn) in an isolated brainstem-spinal cord preparation from newborn rats with bath application of N-methyl-D-aspartate (NMDA). Since natural sucking consists of well-coordinated rhythmical activities of the tongue, face, and jaw muscles, it is assumed that the trigeminal (V) and facial (VII) motoneurons would show rhythmical activities during the RSA.In this study, I tried (1 ) to induce rhythmical activities in these nerves during NMDA application, and (2) if they are induced, to locate the structures essential for generation of the NMDA-induced rhythmical activities. Experiments were performed on brainstem-spinal cord preparations isolated from newborn mice with or without the orofacial structure. Neural activities were recorded from each nerve with suction electrodes. Movements of the tongue and jaw were recorded with a CCD camera system. It was found that (1) bath application of NMDA induced RSA in the V, VII, and XII ns, (2) it also induced rhythmical sucking-like movements of the jaw and tongue, and (3) after complete transections of the brainstem between the V and VII ns as well as the pontomedullary junction, NMDA still induced the RSA in the V, VII, and XII ns, These results demonstrate that separate rhythm generators for the NMDA-induced activities are located at the respective levels in the brainstem, and that the reorganization of the CPG(s) itself would be necessary for the conversion from sucking to mastication.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Katakura N,Nakajima M,Nakamura Y: "Analysis of impaired sucking responses of NMDA receptor e2 subunit mutant mice in in vitro brainstem preparations"Jpn J Physiol. 49(Suppl). S161 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura Y,Katakura N,Nakajima M: "Generation of rhythmical food ingestive activities of the trigeminal, facial, and hypoglossal motoneurons in in vitro CNS preparations isolated from rats and mice"J Med Dent Sci. 46. 63-73 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中島美鈴: "三叉・顔面・舌下神経運動ニューロンの吸啜リズム発生器の脳幹における分節状配列"口病誌. 66. 88-97 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中島美鈴,片倉伸郎: "吸啜リズム発生器の脳幹における分節状配列"口病誌. 66. 296 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katakura N,Ohno T: "Rhythmic membrane potential changes of hypoglossal motoneurons during NMDA-induced sucking-like activity in an en bloc brainstem preparations isolated from newborn mice"Neurosci Res. 24(Suppl). S150 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 片倉伸郎: "摘出脳幹-脊髄標本による神経回路の同定"脳の科学. 22. 79-83 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katakura N,Nakajima M,Nakamura Y: "Neurobiology of Mastication-from Molecular to Systems Approach"Elsevier Science BV, Amsterdam. 525 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katakura, N.: "Ontogenetic analysis of branstem mechanisms of ingestive activities in vitro."Symposium on Neurobiology of Mastication-From Molecular to Systems Approach. (eds Nakamura, Y.and Sessle, B.J.), Elsevier. 312-326 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katakura, N.: "Analysis of Impaired Sucking Responses of NMDA Receptor e2 Subunit Mutant Mice in In Vitro Brainstem Preparations"Jpn. J.Physiol.. 49 (Suppl). S161 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hokazono, T., Katakura, N., Umino, M.and Nakamura, Y.: "Effects of spinal noxious stimulation on trigeminal reflex responses in an in vitro brainstem-spinal cord preparation from newborn rats."Jpn. J.Physiol.. 49 (Suppl). S149 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, Y.: "Generation of rhythmical food ingestive activities of the trigeminal, facial, and hypoglossal motoneurons in in vitro CNS preparations isolated from rats and mice."J.Med. Dent. Sci.. 46. 63-73 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katakura, N.and Ohno, T.: "Rhythmic membrane potential changes of hypoglossal motoneurons during NMDA-induced sucking-like activity in an en bloc brainstem preparations isolated from newborn mice."Neurosci. Res.. 24 (Suppl). S150 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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